五加补骨方及阿仑膦酸钠对模拟失重大鼠骨丢失干预比较  

Comparison of effects of Wujia Bugu decoction(五加补骨方) and Alendronate sodium on protection the bone loss of hindlimb unloaded rats

作　　者：傅骞[1] 胡素敏[1] 杨佳佳[1] 郝喜娟[1] 朱斌[1] 王倩[1] 吴峥嵘[1] 李瑾[2] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]北京积水潭医院骨密度室

出　　处：《中国骨伤》2010年第7期524-528,共5页China Journal of Orthopaedics and Traumatology

基　　金：国家自然科学基金(30500663);教育部优秀人才支持计划(NCET-06-0124);北京市科技新星(A)类计划(2006A49)

摘　　要：目的:比较五加补骨方及阿仑膦酸钠对3周尾吊模拟失重大鼠前后肢骨胳及肌肉丢失的干预作用。方法:自2009年3月至5月,6周龄雄性Wistar大鼠40只,按体重用随机区组法分为:五加补骨方组(HUC)、阿仑膦酸钠组(HUA)、空白组(CON)、模型组(HU),每组10只。动物实验周期为4周,HUC组全程给予五加补骨方(含刺五加、熟地黄、怀牛膝、牡蛎等)水煎剂,按体重10ml/kg剂量每日灌胃1次,药液浓度0.704g/ml;HUA组全程给定量阿仑膦酸钠片溶解混悬剂灌胃,按体重10ml/kg剂量每周灌胃1次,药液浓度0.9mg/ml;CON组和HU组均以上述方法全程给予蒸馏水。自第2周始,HU、HUC、HUA组均以尾部悬吊模拟失重3周。第4周末处死大鼠,分别测量血清钙、磷含量及碱性磷酸酶活性(ALP),肱骨和股骨骨密度(BMD)、肱骨和胫骨生物力学性能(biomechanical property)以及肱二头肌和腓肠肌重量指数。结果:与CON组比较,HU组血清Ca显著升高(P<0.05),后肢BMD、力学性能及肌肉指数均显著降低(P<0.01);与CON组比较,HUA组血清Ca显著升高(P<0.05)。HUC组血清ALP显著高于其他3组(P<0.01)。与HU组比较,HUC组及HUA组股骨BMD均显著升高,胫骨最大载荷、最大桡度及弹性载荷均有升高趋势;与HU组相比,HUC组及HUA组腓肠肌萎缩分别缓解12.5%和50%(P>0.05),肱骨BMD均无显著变化,而HUA组肱骨最大桡度(P<0.01)及弹性桡度(P<0.05)均较HU组有显著降低。结论:中药复方和阿仑膦酸钠均可有效抑制模拟失重造成的后肢骨及肌肉丢失,改善其力学结构。中药复方在缓解上肢力学性能改变方面表现出一定优势。在治疗航天失重骨丢失类疾病上,五加补骨方与阿仑膦酸钠效果相当。Objective：To compare the effects of Wujia Bugu decoction（五加补骨方）and Alendronate sodium on protecting bone and muscle loss of hindlimb unloaded rats lasting three weeks.Methods：March to May,2009,40 male Wistar rats with age of 6 week,were randomized divided to four groups（10 rats in each group）：hindlimb unloaded group treated with Chinese medicine（HUC）,hindlimb unloaded group treated with alendronate sodium（HUA）,control group（CON）,as well as hindlimb unloaded group（HU）.During the experiment,rats of HUC was given Wujia Bugu decoction（including the Ciwujia,Shudihuang,Huainiuxi,Muli,etc.with the concentration of 0.704 g /ml） 10 ml/kg weight once a day,HUA was given quantitative alendronate sodium slice dissolve suspension（0.9 mg /ml） once a week.CON and HU were given double distilled water.The experiment lasted 4 weeks,from the second to the forth week,rats in HU,HUC,HUA were hindlimb unloaded.All rats were sacrificed at the fourth weekend,the content of Ca,P and the activation of ALP in serum,Bone mineral density（BMD） of humerus and femurs,Biomechanical property of tibia and humerus,as well as the weight index of biceps and sural muscles were measured.Results：Compared with CON,serum Ca of HU was significantly increased（P0.05）,BMD,mechanical properties,muscle index of hindlimb were significantly reduce（P0.01）,the serum Ca of HUA significantly increased（P0.05）.Serum ALP of HUC was significantly higher than other three groups（P0.01）.Compared with HU,femoral BMD of HUC and HUA significantly increased,tibial maximum load,maximum deflection and elastic load had increased tendency;calf muscle atrophy of HUC and HUA was alleviate 50% and 12.5% respectively（P0.05）,humeral BMD had no significant difference,while the maximum deflection（P0.01） and elastic deflection（P0.05） in humerus of HUA were significantly lower.Conclusion：Herbal prescription and alendronate sodium can effectively protect the bone and muscle loss of hindlimb unloaded rats,impr

关 键 词：中草药 阿仑膦酸钠 失重模拟 肌 骨骼 

分 类 号：R977[医药卫生—药品]