化痰祛湿法治疗椎动脉型颈椎病的实验研究  

Experimental study of eliminat dampness resolv phlegm method in treating cervical spondylopathy of the vertebral artery type

作　　者：张纯武[1] 吴剑静[1] 韩艳[1] 李章平[1] 许可[1] 李安乐[1] 

机构地区：[1]温州医学院附属第一医院,浙江温州325000

出　　处：《中国骨伤》2010年第7期534-537,共4页China Journal of Orthopaedics and Traumatology

基　　金：浙江省中医药管理局科技项目(编号:2007CA084)

摘　　要：目的:通过对椎动脉血流速度、血液黏度、脑凋亡细胞以及凋亡相关蛋白的检测,为化痰祛湿法治疗椎动脉型颈椎病及作用机制提供实验依据。方法:60只雄性日本大耳兔,随机分为生理盐水组(A组)、盐酸氟桂利嗪组(B组)、小剂量温胆汤组(C组)、大剂量温胆汤组(D组)、合用组(E组,盐酸氟桂利嗪加大剂量温胆汤)和正常喂养组(F组),每组10只。除F组外其他各组采用注射硬化剂法制作椎动脉型颈椎病动物模型。A、F组:生理盐水20ml·kg-1d-1灌胃;B组:盐酸氟桂利嗪溶液0.8mg·kg-1d-1灌胃(用蒸馏水稀释到液体量为20ml·kg-1d-1);C组:温胆汤按20ml·kg-1d-1灌胃,生药含量为1g/ml;D组:温胆汤按20ml·kg-1d-1灌胃,生药含量为2g/ml;E组:盐酸氟桂利嗪0.8mg·kg-1d-1加生药含量为2g/ml的温胆汤20ml·kg-1d-1(盐酸氟桂利嗪溶于温胆汤中,保证总量20ml·kg-1d-1)。治疗前后分别测定椎动脉血流速度、血液黏度指标,测定完毕后处死并检测脑凋亡细胞和凋亡相关蛋白。采用SPSS11.5软件包进行统计学分析。结果:各组动物模型建立满意,E组对家兔椎动脉血流速度、血液黏度的改善最为理想,且脑细胞凋亡指数及凋亡相关蛋白的表达水平最低。结论:在常规治疗椎动脉型颈椎病的基础上加用化痰祛湿法能更好的改善椎动脉血流速度和血液黏度,其作用机制可能和减少脑细胞凋亡有关。Objective：To detect the velocity and viscosity of blood flow of the vertebral arteries,the apoptotic cell and apoptotic related protein in the brain in order to offer theoretical foundation for the treatment of cervical spondylopathy of the vertebral artery type with the eliminat dampness resolv phlegm method.Methods：Sixty male Japanese big ear rabbits were divided randomly into normal sodium group（A）,Flunarizine group（B）,low dosage Wendantang（温胆汤）group（C）,large dosage Wendantang（温胆汤） group（D）,Flunarizine group combined with large dosage Wendantang（温胆汤） group（E）,normal group（F）.Each group had 10 rabbits.Xiaozhiling injection was injected around the vertebral arteries of rabbits in group A,B C,D,E to make the model of the cervical spondylopathy of the vertebral artery type.Normal sodium（20 ml·kg-1d-1） was apply through intragastric administration in group A,F;Flunarizine（0.8 mg·kg-1d-1）,low dosage Wendantang（温胆汤,1 g·ml-1d-1）,large dosage Wendantang（温胆汤,2 g·ml-1d-1）,Flunarizine combined with large dosage Wendantang（温胆汤） were respectively apply through intragastric administration in group B,C,D,E.The velocity and viscosity of blood flow of the vertebral arteries,the apoptotic cell and apoptotic protein in the brain were detected before and after the treatment.Results：Satisfactory animal model were obtained in group A,B,C,D,E.The rabbits of group E had the most improvement of the velocity and viscosity of blood flow of the vertebral arteries while at meantime,which had the lowest apoptotic index and apoptotic related protein expression in the brain.Conclusion：The routine treatment for the cervical spondylopathy of the vertebral artery combined with eliminat dampness resolv phlegm method could improve velocity and viscosity of blood flow of the vertebral arteries,which maybe relate with reduction of apoptosis in the brain.

关 键 词：燥湿化痰 颈椎病 椎底动脉供血不足 血液流变学 细胞凋亡 中草药 

分 类 号：R274.915.5[医药卫生—中西医结合]