砷中毒病区腐植酸对脂质过氧化反应的影响  

Effects of Humic Acid on Lipid Peroxidation in Arsenosis Prevalent Areas

作　　者：余孝颖[1,2,3] 吕锋洲[1,2,3] 郑宝山[1,2,3] 黄天祥 高荣骏 刘聪桂[1,2,3] 洪清霖 

机构地区：[1]中国科学院地球化学研究所 [2]台湾大学医学院生物化学研究所 [3]台北医学院

出　　处：《中华预防医学杂志》1999年第1期26-29,共4页Chinese Journal of Preventive Medicine

基　　金：国家自然科学基金;中华发展基金;有机地球化学国家重点实验室资助

摘　　要：目的探讨台湾乌脚病的病因。方法在体外进行了砷、腐植酸引发脂质过氧化反应的实验。用ＭＤＡ－ＴＢＡ比色法测定了从中国内蒙砷中毒地区和台湾乌脚病区井水以及贵州高砷煤中提取的腐植酸样品。结果试管内实验发现腐植酸能引起不饱和脂肪酸钠盐产生脂质过氧化反应，但作用较弱且不稳定。０．０５ｍｍｏｌ／Ｌ的Ｆｅ２＋能促进这一反应，而１ｍｍｏｌ／Ｌ的Ａｓ２Ｏ３却不能。各地腐植酸样品因来源、成分和结构不同，引起脂质过氧化反应的程度也不同，发现内蒙腐植酸引起脂质过氧化反应能力最强，其次是贵州样品、台湾样品，最后是商品腐植酸（购自美国Ａｌｄｒｉｃｈ公司）。实验还发现微量铁存在时，商品腐植酸引起的脂质过氧化反应是一个动力学过程，它同时具有分解脂质过氧化产物的能力。结论这一实验结果提示流行病学调查者关注乌脚病病区和高砷、高腐植酸地区环境中铁及其他过渡元素与腐植酸和砷的关系。Objective To explore the causes of blackfoot disease in Taiwan. Methods Experiments to induce lipid peroxidation by arsenic and humic acid were made in vitro.Arsenic was determined for the water sampled from the arsenosis prevalent areas in Inner Mongolia and blackfoot disease prevalent areas in Taiwan and humic acid determined for the extracts of the coal rich in arsenic sampled from Guizhou Province in China by MDA TBA colorimetry. Results Experiments found that humic acid could induce lipid peroxidation caused by sodium salt of unsaturated fatty acids in vitro in a weak and unstable manner,which could be promoted by 0.05 mmol/L of Fe ++ and not by 1 mmol/L As 2O 3. The extent of lipid peroxidation caused depended on the sources,constituents and structure of humic acid samples,those from Inner Mongolia ranked the highest,those from Guizhou and Taiwan the medium and the commercial humic acid(Aldrich Co. in the US) the lowest. The experiments also found that lipid peroxidation caused by commercial humic acid presented a dynamic process in the existence of trace amount of iron,and humic acid could decompose the products of lipid peroxidation. Conclusion The experiments suggested the relations of humic acid and arsenic to iron and other transition elements in the blackfoot disease prevalent areas and areas with an environment rich in arsenic and humic acid.

关 键 词：腐植酸 脂质过氧化反应 砷中毒 乌脚病 病因 

分 类 号：R599.9[医药卫生—内科学]