高温油藏采出液中嗜热产甲烷古菌的分离鉴定  

Isolation and identification of a methanogen from the high temperature oil reservoir water

作　　者：刘海昌[1] 兰贵红[2] 刘全全[1] 张文静[1] 曹毅[2] 邓宇[1] 张辉[1] 

机构地区：[1]农业部能源微生物与利用重点开放实验室,成都610041 [2]四川大学生命科学学院,成都610065

出　　处：《生物工程学报》2010年第7期1009-1013,共5页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(No.40973059)资助~~

摘　　要：为了进一步从高温油藏中发掘新的微生物种质资源,采用Hungate厌氧操作技术从大港油田油井采出水中分离出一株嗜热自养产甲烷杆菌DL-7。生理生化结果显示菌株DL-7只能够利用H2/CO2生长,不利用甲酸、甲醇、三甲胺、乙酸和二级醇类;最适生长温度60℃;最适盐浓度0.8 g/L;最适pH为7.0～7.5;只有在添加酵母粉的培养基中才可以较好生长。16S rRNA序列比对结果显示菌株DL-7与标准株M.marburgensis DSM 2133T(X15364)的16S rRNA基因序列相似性为99.7%。To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60℃, pH 7.0-7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T （X15364）.

关 键 词：油藏微生物 热自养产甲烷杆菌 系统发育分析 

分 类 号：Q93-331[生物学—微生物学]