基于壳聚糖-硅溶胶复合膜固载甲苯胺蓝及纳米金的新型电流型过氧化氢生物传感器的研究  

A novel amperometric hydrogen peroxide biosensor based on TB and GNPs as well as silica sol-gel/CS film as immobilization matrix

作　　者：孙妮[1] 李文娟[1] 袁若[1] 柴雅琴[1] 

机构地区：[1]西南大学化学化工学院重庆市分析化学重点实验室,重庆400715

出　　处：《化学传感器》2010年第2期32-36,共5页Chemical Sensors

基　　金：国家自然科学基金(206705064);教育部重点培育项目(708073);重庆市自然科学基金资助项目(CSTC-2009BA1003)

摘　　要：该文基于有机-无机复合膜和纳米技术研制了一种新型的高灵敏度的电流型过氧化氢（H2O2）生物传感器。首先将壳聚糖（CS）和氨丙基三乙氧基硅烷（APTES）交联制得复合膜（CSHMs）,并以该膜固载甲苯胺蓝（TB）和纳米金（GNPs）,然后将HRP与CSHMs-TB-GNPs混合滴涂在玻碳电极的表面,最后在其表面吸附一层Nafion保护膜,制得Nafion/CSHMs-TB-GNPs-HRP/GCE修饰电极。Nafion膜可以减少HRP的泄漏,同时增强了传感器的抗干扰能力。用紫外吸收光谱法分析了修饰膜成分的组成,用循环伏安法对修饰电极进行了表征,并用计时电流法对H2O2传感器的性能进行了研究。实验结果表明,在最佳实验条件下,H2O2浓度在7.0×10-7~2.3×10-3 mol/L范围内与其还原峰电流呈现良好的线性关系,检测下限为2.4×10-7 mol/L（信噪比3）。A novel method based on inorganic-organic hybrid composite film fabrication and nano-technique was exploited to develop a highly sensitive amperometric hydrogen peroxide（H2O2） biosensor.In this method,the hybrid films（CSHMs） are prepared by cross-linking chitosan（CS） with 3-aminopropyltriethoxysilane（APTES）,which can be readily used as an immobilization matrix to co-entrap mediator and enzymes.In the meantime,gold nanoparticle（GNPs） were immobilized into CSHMs via the wildly present amino groups in CSHMs and toluidine blue conjugation.A model enzyme,HRP,was mixed with the CSHMs-TB-GNPs solution and cast on the surface of glass carbon electodes.Finally,the resulting CSHMs-TB-GNPs-HRP/GCE electrode was covered by a thin layer of Nafion to avoid the loss of HRP in determination and to improve the anti-interferent ability.The eletrode was denoted as Nafion/CSHMs-TB-GNPs-HRP/GCE.UV-visible spectroscopies were used to determine the composition of the film.The characteritic of the resulting biosensor was characterized by cyclic votammetry and chronoamperometry.The linear range for the determination of H2O2 is from 7.0×10-7 to 2.3×10-3 mol/L with a detection limit of 2.4 ×10-7 mol/L at 3σ.

关 键 词：过氧化氢生物传感器 CSHMs 甲苯胺蓝 纳米金 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]