碳纳米管-表面活性剂修饰的H_2O_2酶电极  

A H_2O_2 enzyme biosensor modified by carbon nanotubes and surfactant

作　　者：史汶灵[1] 屈永霞[1] 范小雪[1] 张智慧[1] 黄杉生[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《化学传感器》2010年第2期37-41,共5页Chemical Sensors

基　　金：国家高技术研究发展计划(863计划)课题(2007AA06Z402);上海市科委项目(08520510400)

摘　　要：酶在电极上的固定是酶传感器制备中的重要环节,它直接影响酶传感器的检测性能。该文利用静电吸附的自组装法以碳纳米管为载体将酶固定在电极上来制备酶传感器,该酶传感器制备过程简单、稳定性好、组装到电极表面的酶的量多并可保持其生物活性,可检测浓度在1.0×10-6～5.4×10-5 mol/L范围内的H2O2,最低检测限为5.3×10-7 mol/L。这种制备方法为检测农药残留生物传感器的制备提供了一种新的方法。Immobilization of enzyme on the electrodes surface is an important process in the preparation of the sensor because it impact directly on the performance of the sensor.In this paper,we immobilized enzyme on the electrode using the carbon nanotubules as carrier materials based on electrostatic adsorption.These sensors showed good stability because a lot of enzyme can immobilize on the electrode surface and maintain its biological activity.The method showed good linearly for 1.0 × 10-6 ～ 5.4 × 10-5 mol/L H2O2 with a detection limit of 5.3 × 10-7 mol/L under the optimal conditions.This proposed method can provide a new approach for detecting pesticide.

关 键 词：农药 碳纳米管 酶生物传感器 检测 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]