新型鸭肝炎病毒全基因组序列分析  

Genomic analysis of new serotype strains of duck hepatitis virus isolated in China

作　　者：袁率珍[1] 范书才[1] 李虹[1] 李明义 范根成 姜畔[1] 张兵[1] 范小舟[3] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]青岛易邦生物技术有限公司,山东青岛266032 [3]四川大学华西公共卫生学院,四川成都610041

出　　处：《中国预防兽医学报》2010年第7期507-511,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家科技基础条件平台建设项目(2005DKA21205)

摘　　要：为了解新型鸭肝炎病毒(DHV)基因组变异情况,本研究将广东地区分离到的1株,山东地区分离到的2株与1型DHV(DHV-1)无抗原交叉性的新型DHV(N-DHV)全基因组序列测定的结果进行了分析。结果表明,N-DHV基因组全长7786nt～7788nt,仅有一个ORF,其结构具有典型的微RNA病毒科病毒基因组特征。ORF编码一个2251aa的聚合蛋白,该蛋白经3Cpro切割产生3个结构蛋白(VP0、VP3、VP1)和8个非结构蛋白(2A1、2A2、2B、2C、3A、3B、3C、3D)。与其他N-DHV毒株及DHV-1的抗原性相关VP1蛋白氨基酸序列比对表明,3株病毒之间及与国内分离的N-DHVG株的同源性均在98%以上,与韩国N-DHV同源性均大于92%,与中国台湾地区N-DHV同源性为80.1%～80.9%,与DHV-1的同源性为76.9%～77.3%。3株病毒与韩国N-DHVVP1氨基酸差异位点主要集中在C-端的高变异区。依据微RNA病毒科人肠病毒属血清型分型标准,GD株、SD01株、SD02株与韩国N-DHV和G株属于同一血清型,而与DHV-1和中国台湾地区新型DHV属于不同的血清型。The genomic sequences of 3 new serotype（GD,SD01 and SD02） of duck hepatitis virus in China were analyzed.The full lengh of genomes were from 7 786 nt to 7 788 nt including a single open reading frame（ORF） of 6 756 nt encoding a polypeptide of 2251 amino acid（aa）.The polypeptides were cleaved by 3Cpro into three structural proteins（VP0/VP3/VP1） and eight non-structural proteins（2A1/2A2/2B/2C/3A/3B/3C/3D）.The VP1 amino acid sequences shared more than 98 %,92 % and 80.1 %-80.9 % identities with those of the new serotype DHV in Guangdong（G strain）,Korea and Taiwan,respectively.In contrast,they also showed 76.9 %-77.3 % sequence homology with the antigenically unrelated type 1 DHV.To conclude,the GD,SD01 and SD02 strain could be classified into the same serotype of DHV together with new serotype strains in Korea and G strain based on identity scores of VP1 nucleotide and/or amino acid sequences in Enteroviruses.

关 键 词：鸭肝炎病毒 新血清型 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]