B族链球菌多糖—蛋白质结合物黏膜免疫小鼠血清IgG研究  

Immune responses induced by mucosal immunization with group B streptococcus conjugate vaccine

作　　者：黄莉[1] 王爱华[1] 赵志强[2] 周园[2] 朱保权[1] 

机构地区：[1]兰州大学第二医院儿科,甘肃720030 [2]兰州生物制品研究所

出　　处：《中国预防医学杂志》2010年第7期713-715,共3页Chinese Preventive Medicine

基　　金：甘肃省自然科学基金(096RJZA067)

摘　　要：目的探讨B族链球菌荚膜多糖-破伤风类毒素结合物(GBS-CPS-TT),经不同免疫途径免疫小鼠的可行性及免疫剂量。方法经滴鼻、灌胃、皮下注射途径免疫小鼠,各组均免疫3次,每次免疫间隔时间2周,免疫后1周时采集血清,采用间接酶联免疫吸附实验(ELISA)检测血清中特异性IgG的抗体滴度。应用统计软件SPSS13.0对数据进行统计学分析,组间均数比较采用方差分析。结果 GBS-CPS-TT经滴鼻免疫、灌胃免疫和皮下注射均可以诱导产生特异性血清IgG抗体。IgG抗体滴度分别为:滴鼻5μg组(2.671±0.207);滴鼻10μg组(2.911±0.263);皮下注射5μg组(2.866±0.251);灌胃10μg组(2.851±0.224)。滴鼻10μg组诱导产生的IgG抗体滴度明显高于滴鼻5μg组(P<0.01)。结论 GBS-CPS-TT经滴鼻免疫小鼠后可产生系统性免疫应答。Objective To observe the immunogenicity of GBS-CPS-TT conjugate vaccine at different routes of administration.Methods Thirty NIH mice of each group were immunized with GBS-CPS-TT conjugate vaccine 3 times at an interval of 2 weeks through intranasal,intragastric and subcutaneous routes respectively.The production of IgG against GBS was evaluated by ELISA.Results The serum IgG against GBS was significantly increased after intranasal immunization.The concentration of IgG was significantly higher in the group of 10 μg intranasal immunization than that in the group of 5 μg intranasal immunization（P〈0.01）.Conclusion GBS-CPS-TT conjugate vaccine can induce immune response by intranasal immunization.

关 键 词：B族链球菌 多糖蛋白质结合物 黏膜免疫 

分 类 号：R392[医药卫生—免疫学]