有机磷诱导拟南芥差异蛋白表达及其对酯酶同功酶活性的抑制  

Organophosphorus Pesticides(Ops) Operate to Arabidopsis Thaliana by the Different Expression Protein and Inhibiting the Activity of Esterase Isoenzymes

作　　者：丛靖宇[1] 高仙灵[1] 朱宏[2] 万东莉[1] 李国婧[1] 张镝 杜红[1] 王瑞刚[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]哈尔滨师范大学生命科学学院,哈尔滨150080 [3]内蒙古农牧科学院分析测试中心,呼和浩特010000

出　　处：《中国生物工程杂志》2010年第7期43-48,共6页China Biotechnology

基　　金：内蒙古自然科学基金(200508010503;200711020515);教育部回国留学人员启动基金(2006)资助项目

摘　　要：研究植物响应Ops的分子机理对于建立有机磷生物修复技术具有重大的理论意义和实践价值。以拟南芥为例,研究了有机磷胁迫下酯酶同功酶及其可溶性总蛋白的响应。结果表明,有机磷明显抑制了植物的生长发育,并可通过根部吸收的方式迅速进入拟南芥体内,通过抑制酯酶同功酶活性对植物造成胁迫。同时,发现在拟南芥体内存在1个有机磷诱导下的差异表达蛋白,该蛋白与1,5-二磷酸核酮糖羧化酶/加氧酶(RuBisCO)大亚基高度同源。Plants can tolerate or even degrade organophosphorus （OPs）, yet with little known about the mechanisms. Employing Arabidopsis thaliana, a model plant, the response of the esterase isozymes and the total soluble proteins to OPs stress have been studied. The results showed that OPs could be uptooke by Arabidopsis thaliana roots rapidly into the plant, repressed the activity of esterase isoenzyme without reduction in the enzyme content, promoted the production of one different protien highly homologous to the large-subunit sequence of its own ribulose-1,5-bisphophate carboxylase/oxygenase （RuBisCO） and finally, markedly inhibited the plant development. Also, the negative effects of OPs on the plant enhanced with the increase of OPs amount and treating time. The strongly suggested that esterase isoenzyme. probably was the target enzyme of OPs, like in animal, and the different expression protein may also be involved in the response to OPs.

关 键 词：拟南芥 有机磷 酯酶同功酶 差异表达蛋白 可溶性总蛋白 

分 类 号：X5[环境科学与工程—环境工程]