猪精子冷冻损伤研究进展  

Progress on the Cryodamage of Frozen-thawed Boar Spermatozoa

作　　者：陈晓丽[1,2] 朱化彬[2] 郝海生[2] 杜卫华[2] 赵学明[2] 孙艳艳[1] 胡传活[1] 王栋[2] 

机构地区：[1]广西大学动物科技学院,南宁530004 [2]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193

出　　处：《中国生物工程杂志》2010年第7期86-91,共6页China Biotechnology

基　　金：国家自然科学基金(30671502,30940053);国家“863”计划(2006AA10Z143)资助项目

摘　　要：综述了猪冻存精子顶体、质膜和线粒体的形态学变化及其对精子受精能力的影响,同时分析了冻融精子中所发生的蛋白质、DNA等生物大分子的变化及其可能对冻存精子质量及受精能力的影响,指出冷冻保存过程对精子蛋白质、DNA等生物大分子质和量的影响是精子冷冻损伤的实质,应用先进的蛋白组学进行猪精子冷冻损伤机理研究,有助于深刻揭示冷冻损伤的分子机理,为推动猪精液冷冻保存技术研究取得突破性进展提供理论依据。The morphological changes of acrosome, plasma membrane and mitochondrion of frozen-thawed boar spermatozoa and their effect on the fertilization ability of sperm were reviewed, and the changes of biomacromolecules of frozen-thawed boar spermatozoa such as, protein, DNA, etc, and their influence on the quality and fertility of frozen-thawed boar spermatozoa were also analysed. As a result, the conclusion was drawn that the effect of quality and quantity of biomacromolecules such as protein, DNA, are the cryodamage essence of frozen-thawed spermatozoa in the process of cryopreservation. The application of the advanced proteomics on study of the cryodamage mechanism of frozen-thawed boar spermatozoa will contribute to the elucidation of the molecular mechanism of freezing injury, and will provide rationale support for the technique breakthrough of the cryopreservation of boar semen.

关 键 词：猪 精子 冷冻损伤 

分 类 号：Q71[生物学—分子生物学]