CXCL8和CXCR2在血管内皮细胞趋化外周血循环纤维细胞中的作用  

Role of CXCL8 and Its Receptor CXCR2 in Chemotaxis of Vascular Endothelial Cells to Peripheral Blood Fibrocytes

作　　者：曹义战[1] 李金清[2] 崔磊[2] 雷占军[2] 王小林[2] 李学拥[2] 李跃军[2] 

机构地区：[1]第四军医大学唐都医院急诊科,陕西西安710038 [2]第四军医大学唐都医院整形烧伤科,陕西西安710038

出　　处：《现代生物医学进展》2010年第12期2209-2211,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30872679);陕西省社发公关基金(2008K13-03)

摘　　要：目的:周皮细胞的分化在血管新生过程中具有重要作用,没有周皮细胞及其分泌组建的基底膜的支撑,毛细血管就没有正常的功能。作者以前的工作证明周皮细胞可能来源于外周血循环纤维细胞(PBFC),但血管内皮细胞如何趋化PBFC还不清楚。本实验重点观察CXCL8及其受体CXCR2在血管内皮细胞趋化PBFC中的作用。方法:分离纯化人PBFC后与人微血管内皮细胞(HDMEC)共培养,观察共培养条件下PBFC的形态学改变,并检测PBFC细胞内CXCR2 mRNA表达和HDMEC内CXCL8 mRNA的表达。结果:与HDMEC共培养后,PBFC由梭形向菱形改变;HDMEC内的CXCL8 mRNA水平与PBFC共培养24小时后增高约10倍,培养后48小时仍维持在高水平;PBFC内的CXCR2 mRNA水平在共培养后24小时增高约3倍,且在培养后24小时仍维持在较高水平。结论:CXCL8/CXCR2可能参与了血管内皮细胞趋化PBFC的过程。Objective：The differentiation of pericyte plays an important role in angiogenesis.The newly formed vessels have no physiological function without the support of the basal membrane which is secreted and remodeled by pericytes.Our previous work has proved that peripheral blood fibrocytes（PBFCs） might be one of the origins of pericyte,but the mechanism by which vascular endothelial cells（VECs） recruit PBFCs remains unclear.In this article,the role of CXCL8 and CXCR2 in the chemotaxis of PBFCs to the VECs was investigated.Methods：PBFCs were cocultured with human dermal microvascular endothelial cells（HDMECs）.The morphology of PBFCs was observed under light microscope,and the mRNA expression of CXCL8 in HDMECs and CXCR2 in PBFCs were detected by Realtime PCR.Results：PBFCs was in a slim spindle shape,while turned into wide fusiform shape after co-cultured with HDMECs;the mRNA level of CXCL8 in HDMECs co-cultured with PBFCs for 24 hours increased 10 times higher than the HDMECs cultured alone,and maintained at high level 48 hours after cocluture;the mRNA level of CXCR2 in PBFCs co-cultured with HDMECs for 24 hours was 3 times higher than PBFCs cultured alone,and maintained at high level 48 hours.Conclusion：CXCL8/CXCR chemotaxis axis might play an important role in the chemotactic effect of HDMECs on the PBFCs.

关 键 词：血管新生 外周血循环纤维细胞 血管内皮细胞 趋化 

分 类 号：Q75[生物学—分子生物学] Q78