金樱子对糖尿病鼠肝氧化应激的影响  

Effects of Rosa Laevigata Mickx on Expression of Oxidative Stress in Liver of Diabetic Rats

作　　者：许金华[1] 秦振启[1] 周钰娟[2] 罗玉平[3] 张秋菊[1] 

机构地区：[1]南华大学药学与生命科学学院,421001 [2]南华大学护理学院 [3]南华大学附属第二医院

出　　处：《中国实用医药》2010年第21期4-5,共2页China Practical Medicine

基　　金：湖南省科技厅资助项目(项目编号:06FJ3183)

摘　　要：目的研究金樱子对链脲佐菌素(streptozotocin,STZ)和高糖高脂诱导的实验性糖尿病大鼠肝氧化应激的影响。方法用STZ腹腔注射和饲喂高糖高脂诱导法建立SD大鼠糖尿病模型,随机分为糖尿病模型组和金樱子干预组,同时另设正常对照组和金樱子对照组。24周后处死大鼠,取肝组织,用免疫组化方法检测肝脏组织细胞MCP-1、NF-κB二个因子的表达。结果糖尿病模型组MCP-1、NF-κB蛋白的表达与金樱子干预组比较有差异(P<0.05);金樱子对照组与正常对照组比较无差异(P>0.05)。结论金樱子可以下调实验性糖尿病鼠肝MCP-1、NF-κB的表达,增强其抗氧化能力。Objective To investigate the effects of Rosa Laevigata Mickx on oxidative stress in liver of STZ and high sucrose/high fat feed-induced model in rats with experimental diabetes. Methods The SD rats with diabetes by intraperitoneal injection of STZ and high sucrose/high fat feed were randomly divided into two groups ：diabetes model group ,and diabetes intervened with Rosa Laevigata Mickx. In addition another two groups were made ：normal control group ,control group with Rosa Laevigata Mickx. All groups were sacrificed at the 24th week,liver samples were collected. Protein expression of MCP-1 and NF-κB in liver was detected through immunohistochemistry. Methods The protein expression of MCP-1 and NF-κB in liver of the diabetes model group were higher than those of intervention groups （ P 〈0. 05）. The distinction between the control group and the normal group was not obvious （ P 〉0. 05）. Conclusion Rosa Laevigata Mickx can decrease expression of MCP-1 and NF-κB in liver of diabetes rats and increase antioxidation ability of diabetes rats.

关 键 词：糖尿病 金樱子 肝脏 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]