大鼠大脑皮质中Omi/HtrA2的年龄分布差异性及其可能的意义  

Omi/HtrA2 Age Distribution Difference in Rat Cortex and Its Possible Implication

作　　者：刘静祎[1] 王可[1] 史瑞红[1] 秦华平[1] 高峰[1] 王晔[1] 赵欣[1] 刘慧荣[2] 张策[1] 

机构地区：[1]山西医科大学细胞生理学省部共建教育部重点实验室,山西太原030001 [2]首都医科大学基础医学院病理生理学教研室,北京100069

出　　处：《中国现代医生》2010年第21期9-13,共5页China Modern Doctor

基　　金：国家自然科学基金(30670835);国家自然科学基金(30973160);山西省高等学校科技项目(20091115);太原市大学生创新创业项目(091222020)

摘　　要：目的探讨年龄因素影响下大鼠大脑皮质细胞中Omi/HtrA2的表达差异及其与神经细胞凋亡的关系。方法采用正常成年及正常老年大鼠大脑皮质组织,经过Caspase-3、8、9、12活性检测和TUNEL法检测凋亡细胞;Western blot法检测Omi/HtrA2蛋白含量变化;Real-timePCR法检测Omi/HtrA2 mRNA含量变化。结果衰老大鼠大脑皮质细胞凋亡明显高于成年大鼠,具体表现为:前者Caspase-3的比活性显著升高,阳性细胞凋亡数显著增高,与后者相比均有统计学差异(P<0.05)。与成年大鼠相比,衰老大鼠大脑皮质Caspase-9的比活性显著升高,二者相比差异有统计学意义(P<0.05);与成年大鼠相比,衰老大鼠大脑皮质Omi/HtrA2蛋白表达水平显著升高,二者相比差异有统计学意义(P<0.01);与成年大鼠相比,衰老大鼠大脑皮质Omi/HtrA2的mRNA水平也显著升高,二者相比差异有统计学意义(P<0.01)。结论衰老大鼠大脑皮质凋亡水平增高,可能与Caspase-9途径活性增高以及Omi/HtrA2的基因和蛋白质表达水平增加有关。Objective To investigate the influence of the age factor in the rat cerebral cortex cells on the Omi/HtrA2 expression difference and its correlation with neuronal apoptosis. Methods The apoptotic cells from normal adult and normal aged rat cerebral cortex by using caspase3, 8,9 and 12 activity detection and TUNEL assay. The Omi/HtrA2 protein content was detected by using Western blot and the Omi/HtrA2 mRNA content by Real-Time PCR. Results The aging rat cerebral cortex apoptotic cells were significantly more than those in adult rats, the former being significantly higher than the later in Caspase-3 activity and the positive cells, with a significant difference（P〈0.05）. Compared with adult rats, the aging rat cerebral cortex Caspase-9 activity was obviously elevated, with a significant difference between the two（P〈0.05）. Compared with adult rats, the aging rat cerebral cortex Omi/HtrA2 protein expression level was significantly increased, with a significant difference between the two（P〈0.01 ）.Compared with adult rats, aging rat cerebral cortex Omi/HtrA2 mRNA level was significantly increased （P〈0.01）. Conclusion The increased apoptosis level of the aging rat cerebral cortex may be related to the increased caspase-9 activity and the increased expression of gene and protein of Omi/HtrA2.

关 键 词：OMI/HTRA2 衰老 神经细胞 凋亡 

分 类 号：R339.38[医药卫生—人体生理学]