NCOA1基因多态性与邵伯鸡父系公鸡精液品质相关性分析  

Association between SNPs of NCOA1 and semen quality in male line rooster of Shaobo commercial breed

作　　者：赵振华[1] 黎寿丰[1] 黄华云[1] 丁余荣[1] 张静[1] 

机构地区：[1]中国农业科学院家禽研究所,扬州225003

出　　处：《安徽农业大学学报》2010年第3期445-448,共4页Journal of Anhui Agricultural University

基　　金：国家"十一.五"支撑计划(2008BADB2B07);博士基金(JQ2009002)共同资助

摘　　要：采用PCR-SSCP技术检测优邵伯鸡父系公鸡NCOA1基因的SNPs,并分析其与精液品质的关联性。结果表明,第3外显子区域10155007位点发生了T→A突变,检测到AA、AB、BB 3种基因型;第10外显子区域108273257位点发生了T→C突变,检测到SS、ST、TT3种基因型;第12外显子区域108273423位点发生了C→T突变,检测到CC、CD、DD 3种基因型。AA型个体的精液量显著高于BB型个体(P<0.05),精子密度显著高于AB型个体(P<0.05),但AA型个体与BB型个体间精子密度无显著差异(P>0.05)。CC、CD、DD型个体间精液量差异均不显著(P>0.05),但CC型个体的精子密度显著高于DD型个体(P<0.05)。SS、ST、TT基因型个体间的精液量及精子密度均无显著差异(P<0.05)。上述所有基因型个体间的精子畸形率及精子活力差异均不显著(P>0.05)。AACC基因组合型个体的精液品质最好,其在精液量、精子密度方面均显著地优于BBDD基因组合型个体(P<0.05)。研究结果显示,NCOA1基因可能是影响公鸡精液品质性状的主效基因或与控制该性状的主效基因连锁,能够作为候选基因用于精液品质的分子标记辅助选择。Single nucleotide polymorphisms（SNPs） of nuclear receptor co-activator1gene（NCOA1） of sire line of Shaobo commercial breed were detected by using PCR-SSCP and sequencing reaction and its associations with semen quality was done.Three SNPs were detected;T/A substitution at position 10155007 in exon 3 and resulted in 3 genotypes of AA,AB and BB;C/G substitution at position 108273257 in exon 10 and resulted in 3 genotypes of CC,CD and DD;C/T substitution at position 108273423 exon 12 and 3 genotyes of SS,ST and TT were found.AA genotype had more semen volume than that of BB（P0.05）,and it was significantly higher than AB at sperm density（P0.05）.No difference was found between CC,CD and DD（P0.05） at semen volume,but CC was significantly higher than DD at sperm density（P0.05）.Sperm volume and sperm density among genotypes of SS,ST and TT were of no significant significance（P0.05）.There were no significant differences at the sperm motility and the rate of abnormal sperm between all different genotype and haplotype（P0.05）.The haplotype effect of AACC was higher than the single genotype effect and significantly higher than BBDD at sperm density and semen volume（P0.05）.We tentatively conclude that NCOA1 gene is the major gene affecting semen quality or linked to the major gene,and the mutation could be used as the molecular genetic marker to select Shaobo rooster for high semen quality.

关 键 词：公鸡 NCOA1基因 SNPS 精液品质 

分 类 号：S831.2[农业科学—畜牧学]