正交试验优化提取厚朴酚及其HPLC分析方法研究  

Study on Optimization of Magnolol Extraction by Orthogonal Design and HPLC Analysis

作　　者：孔玲[1] 张以阁[1] 荣俊冬[1] 侯孝明[1] 赫春长[1] 郑郁善[1] 

机构地区：[1]福建农林大学林学院,福建福州350002

出　　处：《西南林学院学报》2010年第4期24-27,共4页Journal of Southwest Forestry College

基　　金：福建省科技厅重大科技专项资助项目(2004YZ02-05);福建省科技厅重点项目资助(2009Y0003)

摘　　要：在水浴辅助条件下利用超声处理,提取厚朴中酚类物质,选出最佳方案。采用正交试验设计,HPLC测定厚朴中厚朴酚与和厚朴酚的含量,色谱条件SinoChrom ODS-BP5μm4.6 mm×150 mm色谱柱,流动相为甲醇-水(体积比78∶22),流速为1.0 mL/min,检测波长294 nm,柱温30℃,进样量20μL。在乙醇浓度为80%,浸泡时间50 min,浸泡温度50℃,超声时间为20 min的条件下,酚类物质分离好,提取率高。The schemes for extracting phenolic materials from Magnolia officinalis were studied by orthogonal design under the condition of water bath and ultrasonic treatment.The results showed that the optimal scheme to extract magnolol and honokiol was as follows.The chromatographic conditions included that the SinoChrom ODS-BP（4.6 mm×150 mm,5μm） was used as chromatographic column by HPLC isolation;the methanol water（78∶22） as mobile phase;the flow rate was 1.0 mL/min,the detection wavelength was 294 nm,the column temperature was 30℃ and the injection volume was 20 μL.The highest extracting rate for the phenolic materials and the best separation effect were obtained under the conditions of 80% ethanol as the reagent with 20 min ultrasonic treatment,soaking for 50min at 50℃.

关 键 词：厚朴酚 和厚朴酚 超声法 水浴 HPLC 

分 类 号：S718.43[农业科学—林学]