银花苦丁茶饮料对烫伤大鼠肾脏的保护作用  

The Protective Effect of Honeysuckle Immature Chinese Holly Leaf Beverages to the Kidney of the Scalded Rats

作　　者：柳洁[1] 肖聪颖[1] 汪冶[1] 陈良富[1] 

机构地区：[1]怀化医学高等专科学校组织学与胚胎学教研室,中医药研究中心,湖南怀化418000

出　　处：《怀化医学高等专科学校学报》2010年第1期23-28,共6页

基　　金：湖南省科技厅社会发展支撑计划项目（No.2008SK3034）.科技部中医药行业科研专项中药类资助项目（No.200807020）

摘　　要：目的观察银花苦丁茶饮料对烫伤大鼠肾脏微细结构的影响，评价其对肾脏的保护作用。方法将40只健康SD大鼠随机分成对照组与实验组，每组20只，将两组大鼠制成烫伤病理模型。实验组大鼠于烫伤后服用银花苦丁茶饮料，对照组饮用生理盐水。同时将实验组与对照组分别随机分成4小组，分别于烫伤后2h、4h、6h、8h分组处死大鼠，开腹取肾并制成石蜡切片，为显示肾实质细胞的结构变化与肿瘤坏死因子α表达，切片行HE染色与免疫组织化学SABC法染色，光镜观察结果并照相。免疫组织化学图像用Mimics10．01进行分析，并用SPSS13．0进行统计处理。结果两组大鼠的肾实质与间质均有不同程度的损伤与炎症性改变。病变主要表现在肾实质，近曲小管最明显，血管球扩张、充血与出血，肾小囊扩大。肾小管上皮内可见不同程度的变质细胞：胞质中有散在深红色颗粒的为颗粒变性细胞；胞质中有红色絮状物的为轻度水样变性细胞；胞质中有大小不等空泡的为重度水样变性细胞；细胞呈单一大空泡的为气球样变细胞；胞质完全消失的为裸核细胞。对照组肾实质的炎症与损伤发生时间早，范围广，病变程度重，进展迅速。烫伤后，随着时间推移，肾血管球充血加重，球内毛细血管极度扩张与出血，肾小囊腔内出现红细胞。肾小管的病变由近曲小管逐渐向远曲小管扩展，最终累及集合小管。肾小管上皮细胞由颗粒变性逐渐演变为水样变性与气球样变，最终形成裸核细胞。烫伤后第8h，病变仍继续发展。实验组病变程度轻，病变范围局限，病情发展缓慢。肾血管球充血而无出血。肾小管的主要病变为上皮细胞颗粒样变。烫伤后第8h，病变停止进展。在对照组与实验组，血管球基膜、肾小管上皮均有TNFα表达，染成棕色。对照组TNFα的表达时间早，阳性�Objective To observe the influence of honeysuckle immature Chinese holly leaf beverages on the kidney microstructure and to estimate its protective effect on the kidney. Methods 40 healthy SD rats were divided at random into the contrast group and the experiment group, 20 for each group. According to the length of scalding, the two groups were subdivided into four groups respectively. The experiment group were given the honeysuckle immature Chinese holly leaf beverages after scalding, while the contrast group were given the physiological saline. Both groups were made into the scalding pathology models, put to death separately within 2, 4, 6, or 8 hours after scalding. And the kidneys were taken out and made into paraffin sections. In order to get structural changes of renal parenchymal cells and TNFα expression, slices were stained with HE and immunohistochemical SABC, and they were observed and taken pictures with light microscope. The immunohistochemistry images were analyzed with Mimics10.01 and the statistical treatment was completed with SPSS13.0. Results In the renal stroma and parenchyma of both groups there were different damages and inflammatory changes. The major diseased region was proximal convoluted tubule were the most obvious. The hyperemia, and the capsular spaces were enlarged. The different degenerate ce cells that had red granules in cytoplasm were granule degenerating the renal parenchyma and the pathological changes of hemorrhage, swelling of the glomeruli could be seen lls could be seen in the renal tubular epithelium. The cells. Those that had red floccules in cytoplasm were mild hydropic degenerating cells. Those that had vacuoles of various sizes in cytoplasm were severely hydropic degenerat ing cells. Those that looked like large bubbles were ballooning degeneration cells. Those whose cytoplasm disappeared were naked nucleus cells. As for the contrast group, the inflammation and damage of renal occurred early with extensive coverage, degree of lesion weight and rapid progress. After

关 键 词：金银花 苦丁茶 烫伤 肾 保护 

分 类 号：R977.15[医药卫生—药品]