β-羟丁酸对体外培养牛肝细胞肉碱脂酰基转移酶-Ⅰ转录和翻译水平的影响  

Effect of β-Hydroxybutyrate on Transcription and Translation Levels of Carnitine Palmityl Transferase-Ⅰ in Bovine Hepatocytes Cultured In Vitro

作　　者：徐闯[1] 张日和[2] 夏成[1] 张洪友[1] 王哲[2] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319 [2]吉林大学畜牧兽医学院,长春130062

出　　处：《中国生物制品学杂志》2010年第7期714-716,共3页Chinese Journal of Biologicals

基　　金：国家自然科学基金项目(30871897);黑龙江省青年基金(QC2009C32);黑龙江省农垦总局项目(HNKXIV-08-01-09);黑龙江省教育厅项目(11551312);黑龙江八一农垦大学博士启动基金资助(校启B2009-3)

摘　　要：目的检测不同浓度β-羟丁酸(β-hydroxy butyrate,BHBA)对体外培养牛肝细胞肉碱脂酰基转移酶Ⅰ(Carnitine palmityl transferase-Ⅰ,CPT-Ⅰ)转录及翻译水平的影响。方法分别采用荧光定量PCR法和ELISA法,检测不同浓度BHBA(0、0.3、0.6、1.2、2.4、4.8mmol/L)对体外培养牛肝细胞CPT-I基因和蛋白表达的影响。结果随着BHBA浓度的增加,肝细胞CPT-Ⅰ的转录和翻译水平均下降。当BHBA浓度大于1.2mmol/L时,肝细胞CPT-I的转录和翻译水平下降更显著。结论高浓度的BHBA可显著抑制体外培养牛肝细胞CPT-I的表达,可能减少肝细胞脂肪酸氧化代谢。Objective To investigate the effect of various concentrations of β-hydroxybutyrate（BHBA）on the transcription and translation levels of carnitine palmityl transferase-Ⅰ（CPT-Ⅰ）in the bovine hepatocytes cultured in vitro.Methods The transcription and translation levels of CPT-Ⅰ gene in bovine hepatocytes cultured in vitro after treatment with various concentrations（0、 0.3、0.6、1.2、2.4 and 4.8 mmol /L）of BHBA were determined by fluorescent quantitative PCR and ELISA respectively.Results Both transcription and translation levels of CPT-Ⅰ gene decreased with the increasing BHBA concentration,while decreased remarkably when the BHBA concentration was more than 1.2 mmol /L.Conclusion The BHBA at a high concentration inhibited the expression of CPT-Ⅰ in bovine hepatocytes cultured in vitro significantly,and might decrease the fatty acid oxidation.

关 键 词：3-羟基丁酸 肉碱脂酰基转移酶 肝细胞 

分 类 号：Q251[生物学—细胞生物学]