甜瓜PGIP基因的克隆及表达初步分析  

Cloning and Expression of PGIP Gene from Cucumis melon L.

作　　者：段玉娟[1] 郭庆勋[1] 刘志伟[1] 宋阳[1] 怀凤涛[2] 

机构地区：[1]吉林大学植物科学学院,吉林长春130062 [2]东北农业大学园艺学院,黑龙江哈尔滨150030

出　　处：《华北农学报》2010年第3期38-42,共5页Acta Agriculturae Boreali-Sinica

基　　金：吉林大学农学部博士启动基金项目(430505010203);长春市科技局国际合作计划项目(2D5070046202)

摘　　要：以甜瓜叶片基因组DNA为模板,PGIP基因保守序列设计引物,PCR扩增到1条全长978 bp的目的片段。该基因包含有1个完整的开放阅读框,没有内含子,编码326个氨基酸,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列。序列比对表明:该基因编码的氨基酸序列与GenBank中的PGIP基因氨基酸序列(AAP41199)同源性为100%;RT-PCR表达分析表明,该基因在甜瓜叶、茎、根、果实中表达,在花中不表达。为植物分子抗病育种提供了1条基因资源。A target fragment with a full length of 978 bp was amplified with genomic DNA of Cucumis melon L leaves as the templates and the conservative sequences of PGIP gene as the primers.This sequence had a full open reading frame encoding the polygalacturonase-inhibiting protein.The total ORF were comprised by 975 bp of deoxynu-cleotide encoding 325 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Se-quencing analysis showed that it was 100% identical with the sequences of PGIP gene which had been cloned.RT-PCR analysis showed that the PGIP gene was expressed in roots,stems,leaves and fruits of cucumis melon L.As a re-sult,a gene resource was provided for molecular breeding of plants.

关 键 词：甜瓜 PGIP基因 基因克隆 表达 

分 类 号：Q786[生物学—分子生物学]