亚比棉×海岛棉杂种F_1的遗传分析  

Genetic Analysis of Hybrid F_1 from (G. arboreum ×G. bickii) ×G. barbadense

作　　者：渠云芳[1] 黄晋玲[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《华北农学报》2010年第3期64-67,共4页Acta Agriculturae Boreali-Sinica

基　　金：山西省科技攻关项目(20080311001-1);山西农业大学科技创新基金(2004082)

摘　　要：为了验证杂种的真实性,利用常规压片法和RAPD技术对亚比棉×海岛棉杂种F1及其亲本进行了研究。细胞学研究表明,3种杂种(亚洲棉×比克氏棉)×海岛棉的核型公式为:2n=4x=52=44m(2SAT)+8sm(2SAT),相对长度变异范围为6.91%～1.99%,臂比值为1.01～2.31之间,平均臂比为1.37,核型类型为2B。RAPD分析表明,亚比棉×海岛棉杂种F1共扩增出60条DNA带,其中具亚洲棉特异带的占总带数的3.3%;来自比克氏棉的带占8.3%;具海岛棉特异带占18.3%;来自亚比棉的特异带占10%;杂种的特异带占8.3%。To veritify the truth of hybrid,（G.arboreum × G.bickii） × G.barbadense and its parents were studied through conventional compression method and RAPD technology.The result of cytology showed that the karyotype foumula of tri-hybrid（G.arboreum × G.bickii） × G.barbadenseis 2n =4x = 52 = 44m（2SAT） ＋ 8sm（2SAT）,the range of relative length is 6.91-1.99%,the range of arm ratios is 1.01-2.31,mean arm ratios is 1.37.The type of karyotype is 2B.The analysis of RAPD showed： A total of sixty DNA bands were amplified in（G.arboreum × G.bickii） × G.barbadense,3.3% DNA bands came from the specifical bands of G.arboreum,8.3% was from the spefical bands of G.bickii,18.3% DNA bands came from the specifical bands of G.barbadense and 8.3% came from the special bands of tri-specific hybrid.

关 键 词：亚比棉×海岛棉 杂种 遗传分析 

分 类 号：S562.03[农业科学—作物学]