绍兴鸭微卫星DNA遗传多样性分析  

The DNA Genetic Diversity of Shaoxing Duck Populations

作　　者：袁青妍[1] 陶争荣[1] 李国勤[1] 沈军达[1] 陈奕春 陈文标 王德前[1] 虞永亮 田勇[1] 原爱平[1] 卢立志[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [2]缙云县畜牧兽医局,浙江缙云321400 [3]浙江五莲农牧有限公司,浙江缙云321403

出　　处：《华北农学报》2010年第3期73-75,共3页Acta Agriculturae Boreali-Sinica

基　　金：国家"十一五"科技支撑计划项目(2006BAD14B06);浙江省重大科技攻关项目(2005C12005-01);浙江省重大国际合作项目(2006C14015)

摘　　要：采用鸭的6个高度多态且分布在不同连锁群上的微卫星标记,对原种绍兴鸭种群中48个体和青壳II系绍兴鸭种群中34个体分别进行遗传多样性分析,共检测到152个等位基因,每个微卫星座位上等位基因数介于20～30,基因频率分布在0～0.2,在两个种群中每个微卫星标记的杂合度及多态信息含量均在0.8以上。原种绍兴鸭在3个座位上处于Hardy-Weinberg不平衡状态,而青壳II系绍兴鸭在2个座位上处于Hardy-Weinberg不平衡状态,而且它们都在AY493314座位处于Hardy-Weinberg不平衡状态。研究结果表明:原种绍兴鸭与青壳II系绍兴鸭群体内均存在丰富的遗传多样性。Six microsatellite markers with high polymorphisms locating at different linkage groups were used to analyze the microsatellite DNA genetic polymorphisms of the 48 and 34 Shaoxing ducks sampled from element breed population and blue-eggshelled II strain population,respectively.A total of 152 alleles were detected,and the numbers of alleles at each locus varied from 20 to 30.The gene frequencies were 0 to 0.2.Both the hererozygosity（H） and poly-morphic information content（PIC） were more than 0.8 at each microsatellite locus in the two populations.In the ele-ment breed population and blue-egg shelled II strain population,there were 3 and 2 loci,respectively,which were not in Hardy-Weinberg equilibrium,and they were all at AY493314 locus.The results revealed that the genetic variabili-ties in the element breed and blue-eggshelled II strain populations analysed were rich.

关 键 词：绍兴鸭 遗传多样性 微卫星标记 

分 类 号：S834.03[农业科学—畜牧学]