男性不育患者精浆中游离睾酮、游离左旋肉碱水平与精液参数的相关性分析  

Correlation analysis of seminal plasma free testosterone and L-carnitine with semen parameters

作　　者：柳建明[1] 姜辉[1] 洪锴[1] 赵连明[1] 唐凌峰[1] 刘德风[1] 马潞林[1] 赵一鸣[2] 

机构地区：[1]北京大学第三医院泌尿外科,北京100191 [2]北京大学第三医院临床流行病学研究中心,北京100191

出　　处：《第二军医大学学报》2010年第7期767-769,共3页Academic Journal of Second Military Medical University

摘　　要：目的分析男性不育患者精浆中游离睾酮和游离左旋肉碱水平与精子密度、活动率和活力参数间的相关性,探讨二者对男性生育力的影响及在不育症检查和治疗中的作用。方法分别采用化学发光免疫分析法、液相色谱-质谱/质谱联用技术和计算机辅助精液分析系统,测定192例不育男性(正常精液组36例,少精组48例,弱精组60例,少弱精组48例)精浆中游离睾酮、游离左旋肉碱水平及精子密度、活动率、活力等参数。采用SPSS13.0软件包进行统计处理,分析各组游离睾酮和游离左旋肉碱水平的差异以及游离睾酮和游离左旋肉碱水平与精子密度、活动率、活力间的相关性。结果正常精液组游离睾酮和游离左旋肉碱水平高于其他3组(P<0.05)。相关性分析结果显示:精浆中游离睾酮水平与精子密度存在正相关关系(r=0.427,P<0.05),精浆游离左旋肉碱水平与精子密度存在正相关关系(r=0.318,P<0.01),精浆中游离左旋肉碱水平与精子活动率及活力间呈现正相关关系(r=0.641,P<0.01;r=0.568,P<0.01)。结论精浆中游离睾酮水平与精子密度正相关,游离左旋肉碱水平与精子密度、活动率、活力正相关,二者可作为一项有效的生化指标,为男性不育症临床诊治提供参考。Objective To analyze the correlation of seminal plasma free testosterone and free L-carnitine with semen density,vitality,and motility,so as to investigate their effects on male fertility and their roles in the examination and treatment of male infertility.Methods Using chemiluminescence immunoassay method,LC-MS-MS and computer-assisted semen analysis system,we examined the seminal levels of free testosterone,free L-carnitine and sperm density,vitality,and motility in 192 infertile males（normozoospermia,n=36;oligozoospermia,n=48;asthenozoospermia,n=60;and oligoasthenozoospermia,n=48）.The difference of free testosterone and free L-carnitine levels between different groups was analyzed,and the correlation of their levels with semen density,vitality,and motility was also analyzed using the SPSS 13.0 statistical software.Results The levels of free testosterone and free L-carnitine in the seminal plasma of the normozoospermia men were significantly higher than those of others（P0.05）.Correlation analysis showed that there was positive correlation between seminal free testosterone with the sperm density（r=0.427,P0.05）,seminal free L-carnitine with the sperm density（r=0.318,P0.01）,and seminal free L-carnitine with sperm vitality,motility（r=0.641,P0.01;r=0.568,P0.01）.Conclusion Seminal free testosterone level is correlated with the sperm density,and seminal free L-carnitine level is correlated with sperm density,motility and vitality;both levels can be taken as a biochemical index for the clinical diagnosis and treatment of male infertility.

关 键 词：男性不育 睾酮 左旋肉碱 精子 

分 类 号：R698.2[医药卫生—泌尿科学]