高效液相色谱法测定乙酰丙酮含量  

Determination of Acetylacetone with HPLC

作　　者：李广梅[1] 郑典模[1] 彭海龙[1] 朱升干[1] 屈海宁[1] 陈优霞[1] 

机构地区：[1]南昌大学环境与化学工程学院,江西南昌330031

出　　处：《化学世界》2010年第7期398-400,共3页Chemical World

摘　　要：探索了建立高效液相色谱法测定乙酰丙酮含量。其方法为:采用岛津LC-10AT型高效液相色谱仪,使用Shi madzu VP-ODS(150 mm×4.6 mm,4.6μm)色谱柱,以甲醇(100%)作为流动相,流速0.3 mL/min,检测波长274 nm,柱温30°C。结果为乙酰丙酮进样量在15.62～156.2 ng范围内与峰面积线性关系良好(r=0.999 9);乙酰丙酮平均回收率(n=6)为100.02%;相对标准偏差(RSD)为1.44%。该方法简便、快速、准确、重复性好,可用于乙酰丙酮含量测定。High performance liquid chromatography（HPLC） method for the determination of acetylacetone was explored.The chromatographic column was Shimadzu VP-ODS（150 mm×4.6 mm,4.6 μm）,the mobile phase was methanol（100%）,the flow rate was 0.3 mL/min,the UV detection wavelength was 274 nm and the column temperature was 30 °C.Within the range of 15.62～156.2 ng,the content of acetylacetone had a good linear relationship（r=0.999 9）,and the average recovery（n=6） was 100.02% with relative standard deviatiom of 1.44%.The method is simple,fast,accurate,and can be used for the content determination of acetylacetone.

关 键 词：高效液相色谱法 乙酰丙酮 含量测定 

分 类 号：TQ224.24[化学工程—有机化工]