益髓灵氯仿提取物诱导HL—60细胞凋亡的初步研究  

Preliminary Study on HL -60 Cellular Wither Induced by Chloroform Extract of Yisuiling

作　　者：陈信义[1] 邵德彬[2] 赵立业[1] 胡凯文[1] 庞大本[2] 池旭生[2] 李玉梅[2] 

机构地区：[1]北京中医药大学东直门医院,北京东城区海运仓100700 [2]中国中医研究院

出　　处：《中医杂志》1999年第4期235-236,共2页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金课题部分内容(批准号:3947863)

摘　　要：本项研究重点进行了益髓灵氯仿提取物、含氯仿提取物的兔血清、含氯仿提取物的兔肝匀浆、足叶乙甙、正常兔血清五组诱导HL—60细胞凋亡效应的实验研究。结果显示,短期液体培养后,取培养的HL—60细胞,经光学显微镜观察与流式细胞仪分析,前四组均见明显的HL—60细胞凋亡现象。其中,含益髓灵氯仿提取物的兔肝匀浆作用最强,与阳性对照药足叶乙甙相似,兔血清作用次之,益髓灵氯仿提取物作用较弱,正常兔血清无明显的诱导HL—60细胞凋亡作用。对于其诱导凋亡机理尚正在研究之中。The experiment was conducted on the actions of 5 kinds of reagents on HL - 60 cellular wither, viz. Yisuiling chloroform extract, rabbit serum chloroform extract, rabbit liver paste chloroform extract, Etoposide, and normal rabbit serum. Results revealed that after fluid incubation for a short period, the HL-60 cultured cells showed no wither in the former 4 groups under microscope and flowing cytometer. Among them, action was strongest in rabbit liver paste of Yisuiling extract group, similar to the positive control of Etoposide. Followed by rabbit serum, and the action was weak in Yisuiling chloroform extract. There was no obvious action of induced wither by normal rabbit serum. The mechanism of induced wither is still under investigation.

关 键 词：白血病 益髓灵 氯仿提取物 HL-60 中医药疗法 

分 类 号：R273.370.5[医药卫生—中西医结合] R273－361[医药卫生—中医肿瘤科]