高效液相色谱法测定左乙拉西坦血药浓度  

Therapeutic Drug Monitoring of Levetiracetam by HPLC-UV

作　　者：王颖慧[1] 魏敏吉[1] 王云秀[1] 王丽[1] 

机构地区：[1]北京大学第一医院,北京100034

出　　处：《儿科药学杂志》2010年第4期34-36,共3页Journal of Pediatric Pharmacy

摘　　要：目的:建立左乙拉西坦HPLC方法学。方法:100μL血清加入内标后二氯甲烷去蛋白处理,色谱柱为Attima硅胶柱(5μm,4.6mm×150mm);流动相为磷酸盐缓冲液(50mmol,pH4.5)和乙腈(96∶4,V/V),流速1mL/min;检测波长210nm,检测灵敏度0.0001AUFS;柱温37℃;进样量10μL。结果:左乙拉西坦在4.5min出峰,内标在6.5min出峰,无干扰;线性关系良好,相关系数R=0.9999,线性范围1.25～160μg/mL。结论:该方法准确,可用于临床LEV血清浓度的常规检测,促进临床合理用药以及个体化治疗。Objective：In order to develop an HPLC method with Ultraviolet Detection（HPLC-UV）for monitoring drug plasma levels in patients treated with levetiracetam（LEV）.Methods：Plasma sample（100 μL）pretreatment was based on simple deproteinization by dichloromethane spiked with the internal standard（ucb17025）.HPLC analysis was carried out on an Attima Si column（4 μm,150 mm×4.6 mm）.The mobile phase was a mixture of potassium dihydrogen phosphate buffer（50 mmol,pH 4.5）and acetonitrile（94∶6,V/V）at an isocratic flow rate of 1.0 mL/min.The UV detector was set at 210 nm.Results：Calibration curves were linear（mean correlation coefficient was 0.9999）within a range of 1.25~160 μg/mL.The chromatographic run lasted 8 min.Conclusions：This assay is proved to be very practical in our therapeutic drug monitoring and can be applied to patients with epilepsy.

关 键 词：左乙拉西坦 高效液相色谱法 治疗药物监测 

分 类 号：R969.3[医药卫生—药理学]