血清高密度脂蛋白胆固醇均相法测定试剂的研制  

Homogeneous assay for measuring serum high density lipoprotein cholesterol

作　　者：高德路[1,2] 张世俊 李培瑛[1,2] 

机构地区：[1]解放军第三○五医院检验科 [2]卫生部北京老年医学研究所

出　　处：《中华医学检验杂志》1999年第2期104-108,共5页

摘　　要：目的研制高密度脂蛋白胆固醇均相法测定的试剂。方法利用多聚体和聚阴离子在表面活性剂的作用下对脂蛋白进行选择性遮蔽，仅使高密度脂蛋白中的胆固醇参与胆固醇反应。结果研制的试剂（Ｙ）与推荐方法ＤＳ５０Ｍｇ（Ｘ１）和ＰＴＡＭｇ沉淀法（Ｘ２）相关良好，分别为Ｙ＝１．０４０Ｘ１－０．０６３，ｒ＝０．９８８３；Ｙ＝０．９４４Ｘ２＋０．０７３，ｒ＝０．９６５７。在ＨＤＬＣ浓度为２．８４ｍｍｏｌ／Ｌ以下线性良好，ｒ＝０．９９８２，ＨＤＬＣ低、中、高值血清标本的批内和批间（ＣＶ）值分别为２．０７％、１．８１％、０．８０％和２．７７％、１．９７％、１．７３％，特异性好，平均回收率为９８％，胆红素、血红蛋白等干扰不明显。结论ＨＤＬＣ均相法测定结果的准确性与精密度符合临床要求。Objective To prepare the homogeneous assay reagents for measuring high density lipoprotein cholesterol and its analytical performance. Methods Selective inhibition assay principle was applied in the enzymatic determination of HDL cholesterol. appropriate quantities of polymer, polyanion, surfactants, and cholesterol enzyme reagents were used in the two regent solutions.Results The directly determined HDL C values with the home prepared reagent ( Y ) closely correlated with those obtained by precipitation with DS Mg( X 1)( Y = 1.040 X 1 0.063) and PTA Mg ( X 2)( Y =0.944 X 2+0.073) methods. The assay was linear up to at least 2.84 mmol/L( r =0.998 2).The within run CV and between run CV at low、middle、high HDL C concentration specimens were 2.07%, 1.81%, 0.80%, and 2.77%, 1.97%, 1.73% respectively. The average recovery rate of the added pure HDL was 98%. Interference by bilirubin and hemoglobin was insignificant. Conclusion The precision and accuracy of HDL C determination with this homogeneous assay method can fulfill the clinical requirement.

关 键 词：胆固醇 均相法 血清诊断 高密度脂蛋白 

分 类 号：R446.112[医药卫生—诊断学]