姜黄素对喉癌Hep-2细胞增殖及Bcl-2、Bax基因表达的影响  

Effect on the expression of Bcl-2,Bax and on cell proliferation by curcumin in laryngeal cancer cell Hep-2

作　　者：张立[1] 刘剑凯[2] 孙吉凤[1] 邹维[1] 

机构地区：[1]长春医学高等专科学校生物化学教研室,吉林长春130031 [2]吉林大学白求恩医学院生物化学与分子生物学教研室,吉林长春130021

出　　处：《中国实验诊断学》2010年第7期1053-1055,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省教育厅"十一五"科学技术研究项目资助课题(2008-432)

摘　　要：目的探索姜黄素对喉癌细胞增殖的作用以及对细胞Bcl-2和Bax基因表达的影响。方法 Hep-2细胞暴露于含姜黄素(3-25μM)的培养基中,分别培养24h及48h,用MTT法检测对细胞增殖的影响;用RT-PCR法检测Bcl-2和Bax的mRNA表达水平。结果 MTT显示,姜黄素使Hep-2细胞增殖明显降低,并呈时间-剂量依赖性;RT-PCR结果显示,姜黄素使Bcl-2的mRNA表达水平降低,而使Bax的mRNA表达水平升高,并呈剂量和时间依赖性。结论姜黄素对人喉癌Hep-2细胞增殖具有显著的抑制作用。其作用机制可能与其下调Bcl-2基因表达水平及上调Bax基因表达水平,从而诱导细胞凋亡有关。Objective To study effect of curcumin on proliferation and the expression of Bcl-2,Bax in laryngeal cancer cell Hep-2.Methods Hep-2 cells were exposed to curcumin at concentrations from 3 to 25 μM,cultured for 24 h or 48 h.MTT was used to determine cell proliferation.The expression levels of Bcl-2,Bax mRNA were analyzed by RT-PCR.Results MTT showed that the cell proliferation was down regulated by curcumin significantly in a does and time dependent manner;RT-PCR showed that the expression of Bcl-2 was decreased and the Bax mRNA was increased by curcumin.Conclusion Curcumin inhibits the proliferation of laryngeal cancer Hep-2 cells significantly.The possible mechanism is related with curcumin down regulating bcl-2 but up regulating bax.

关 键 词：姜黄素 喉癌细胞 细胞增殖 BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学] R739.65[医药卫生—中医学]