人胎盘因子对小鼠体内抗体分泌的影响  

INFLUENCE OF MICE ANTIBODY SYNTHESIS IN VIVO BY HUMAN PLACENTAL FACTORS

作　　者：田仲萍[1,2] 闫燕华[1,2] 

机构地区：[1]天津医科大学免疫学教研室 [2]天津市第一中心医院血栓与止血研究所流式细胞仪室

出　　处：《免疫学杂志》1999年第2期99-100,共2页Immunological Journal

基　　金：天津市卫生局科技基金

摘　　要：为探索ＨＰＳ（人胎盘培养上清）对小鼠体内ＩｇＭ型抗体分泌的影响；用ＳＲＢＣ（绵羊红细胞）免疫雌性Ｂａｌｂ／ｃ健康小白鼠的同时，腹腔注射不同浓度的ＨＰＳ，免疫第４天检测小鼠血清中ＩｇＭ型抗ＳＲＢＣ抗体的效价。与对照组相比，低浓度（２．５％、５％、１０％）ＨＰＳ对小鼠体内ＩｇＭ型抗体分泌具有促进作用，Ｐ＜０．０１；而高浓度（２０％）ＨＰＳ对其分泌没有影响，Ｐ＞０．０５，说明ＨＰＳ能够调节小鼠体内ＩｇＭ型抗体的分泌。在孕妇体内，胎盘分泌的ＣＫ（细胞因子）如何调节ＩｇＭ型抗体分泌以及对母体耐受胎儿方面有何影响尚待进一步研究。To study the influence of human placental culture supernatants(HPS)on mice IgM antibody synthesis in vivo,the female Balb/c healthy mice were immunized with sheep erythrocyte and at the same time injected i.p.different consentration of HPS.At the fourth day,we collected the venous blood to investigate the antibody production by agglutination test.The results showed that the antibody production significantly increased when injected i.p.low consentration(2.5%、5%、10%)of HPS contrast to the control group.There is no significant difference between experimental group and control group about the antibody production when injected i.p.high consentration(20%)of HPS.It is concluded that the synthesis of mice IgM antibody in vivo can be regulated by HPS.

关 键 词：胎盘培养上清 抗体分泌 凝集试验 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]