血小板生成素糖基化与其体内生物学活性关系的研究  

STUDIES ON RELATIONS OF THROMBOPOIETIN GLYCOSYLATION AND ITS BIOLOGICAL EFFECTS IN VIVO

作　　者：刘丽[1,2] 王颖[1,2] 田生礼[1,2] 芦兴武[1,2] 孟岩 刘立忠[1,2] 谢宝树[1,2] 冷爱军 

机构地区：[1]空军总医院临床分子生物研究中心 [2]东北师范大学细胞与遗传研究所

出　　处：《免疫学杂志》1999年第2期103-105,共3页Immunological Journal

基　　金：国家自然科学基金

摘　　要：为探讨血小板生成素（ＴＰＯ）糖基化与其体内生物学活性的关系，给血小板缺乏症小鼠模型腹腔分别注射相同活性单位酵母系统表达的人ＴＰＯ成熟肽或大肠杆菌表达的人ＴＰＯＮ－端结构域蛋白，连续给药５ｄ，每天一次，末次给药后３ｄ，比较两种ＴＰＯ组动物外周血血小板数量、骨髓巨核细胞培养单位（ＣＦＵ－Ｍｅｇ）、骨髓有核细胞ＤＮＡ含量。结果高、中、低剂量的ＴＰＯ成熟肽组动物的血小板数、巨核细胞集落数和骨髓ＤＮＡ含量均明显高于ＴＰＯＮ－端结构域蛋白的相应组（Ｐ＜０．０１）。显示酵母系统表达的ＴＰＯ成熟肽比大肠杆菌表达的ＴＰＯＮ－端结构域有明显的生物学活性，这可能与它们的糖基化程度及半衰期有关。To investigate the relation of thrombopoietin(TPO)glycosylation and its biological effects in vivo,human TPO mature peptide expressed by Piochia Pastoris or TPO N terminal domain protein expressed by E.coli were injected into thrombocytopenia mice with the same activity unit in 5 days by intraperitoneal injection once for a day.On fourth day after last injection,peripheral platelet number,CFU Meg and DNA content in bone marrow cells were compared in two kinds of TPO.The results showed that platelet number,CFU Meg and DAN content in TPO mature peptide were remarkably higher than that of APO N terminal domain protein in different dosage groups.TPO mature peptide expressed by Pichia Pastoris has significantly higher biological activity than that of TPO N terminal domain protein expressed by E.coli.It may be related to their glycosylation degree and half life.

关 键 词：血小板生成素 糖基化 生物学活性 

分 类 号：Q592.1[生物学—生物化学] Q78