双抗原夹心酶免疫试验对人畜布鲁氏菌抗体检测的研究  

Study on double antigens sandwich Enzyme Immunoassay for detection of brucella specific antibodies in human and animals

作　　者：鲁齐发[1] 张伟[1] 郝宗宇 

机构地区：[1]中国预防医学科学院流行病学微生物学研究所,北京102206 [2]河南省地方病防治研究所

出　　处：《中华流行病学杂志》1999年第2期118-121,共4页Chinese Journal of Epidemiology

摘　　要：目的　为人畜布鲁氏菌病(布病)血清学诊断、监测及流行病学调查提供实用和简便的方法。方法　在首次制备布鲁氏菌(布氏菌)抗原-辣根过氧化物酶结合物的基础上,建立了检测人畜布氏菌抗体的双抗原夹心酶免疫试验(DAgS-EIA,包括DAgS-ELISA及DAgS-DIEA)。用本法及间接ELISA(I-ELISA)、试管凝集试验(SAT)及虎红平板凝集试验(RBPT)等常规血清学方法对从河南省及河北省收集的部分布病患者、可疑患者,布氏菌感染羊只以及布氏菌感染的实验动物血清标本进行了检测。结果　对布病患者及可疑者的检查结果表明,阳性率以DAgS-ELISA最高(60.0%～62.9%),余依次为RBPT(48.6%～58.1%)、I-ELISA(55.6%)、DAgS-DIEA(53.7%)及SAT(44.2%);对布氏菌感染羊的阳性率均为81.8%。结论　上述结果表明,双抗原夹心酶免疫试验检测人畜布氏菌抗体,不仅特异、简便而且仅制备一种布氏菌抗原-酶结合物就可对人及多种动物进行检测。Objective In order to develop practicable methods for serodiagnosis,surveillance and epidemiological surveys in human and animals Brucellosis. Methods A double antigens sandwich Enzyme Immunoassay (DAgS-EIA include DAgS-ELISA and DAgS-DIEA) have been developed under the basis of first production conjugate of Brucella antigen with horseradish peroxidase. Serum samples from diagnosed patients, suspected patients of Brucellosis and sheep infected with Brucella in Henan and Hebei provinces as well as from experimental animals of infected with Brucella in laboratory detected through DAgS-EIA,I-ELISA,RBPT and SAT tests. Results Of the diagnosed and suspected Brucellosis patients, positive rates of DAgS-ELISA,RBPT,I-ELISA,DAgS-DIEA and SAT were 60.0 %- 62.9 %, 48.6 %- 58.1 %, 55.6 %, 53.7 % and 44.2 % respectively; as for sheep with Brucella infection, positive rate of the above mentioned 5 tests were 81.8 %. Conclusion Results showed that DAgS-EIA were not only specific and practicable but may use for Brucella specific antibodies detection in humans and various animals with only one conjugate of Brucella antigen with horseradish peroxidase.

关 键 词：布鲁氏菌病 抗原 抗体 ELISA DAgSEIA 

分 类 号：R516.704[医药卫生—内科学] R446.62[医药卫生—临床医学]