丹参对大鼠肝缺血后再灌注损伤的保护作用  

Protective effect of Salvia on ischemia-reperfusion hepatic injury in rats

作　　者：叶辉[1] 付晓光[1] 陆航[1] 毛东[1] 

机构地区：[1]辽宁医学院附属第一医院普通外科,锦州121001

出　　处：《中华医学杂志》2010年第26期1854-1858,共5页National Medical Journal of China

摘　　要：目的 通过检测丹参对大鼠肝缺血再灌注损伤后核因子-Kappa B（NF-κB）、细胞间黏附因子-1（ICAM-1）表达的影响,观察丹参对大鼠肝缺血后再灌注损伤的保护作用.方法 按Nauta等方法制备大鼠肝脏缺血再灌注模型,随机分为3组：假手术组（S组）、缺血再灌注组（I/R组）、丹参预处理组（P组）,分别于肝缺血再灌后2、8、24 h取材,用免疫组化方法检测肝组织NF-κB、ICAM-1的表达,测肝组织中髓过氧化酶（MPO）浓度评价肝组织中中性粒细胞浸润情况,检测血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平及做光镜、电镜检查反映肝组织细胞损伤程度.结果 I/R组NF-κB A值于再灌注2h（0.418 4±0.039）明显升高,8h（0.308 4±0.028）、24h（0.240 ±0.032）逐渐下降.ICAM-1 A值也于再灌注2h（0.367±0.034）升高,8h（0.451±0.031）达高峰,24h（0.293±0.025）下降.MPO、ALT、AST变化与ICAM-1类似.在P组上述各指标在各时间点均较I/R组低（P〈0.05）,但较S组高（P〈0.05）.ALT、MPO、NF-κB和ICAM-1之间的变化均具有正相关（P〈0.01）;光镜、电镜观察各组之间也具有明显区别.结论 大鼠肝缺血再灌注时刺激NF-κB、ICAM-1的表达参与肝脏缺血再灌注损伤的发生过程,丹参能显著降低缺血再灌注时肝组织中NF-κB、ICAM-1的表达,并有效改善肝缺血再灌注损伤.Objective To detect the expressions of nuclear factor-Kappa B （ NF-κB ） and intercellular adhesion molecule-1 （ICAM-1 ） after hepatic ischemia-reperfusion injury so as to study the protective effect of Salvia in rats. Methods The hepatic ischemia-reperfusion rat model was established by the method of Nauta. The rats were randomly divided into 3 groups： sham operation （ S）, ischemiareperfusion （ I/R） and Salvia preconditioning （P）. At 2, 8, 24 h after ischemia-reperfusion,immunohistochemistry was employed to detect the hepatic expressions of NF-kB and ICAM-1. Measurement of hepatic tissue myeloperoxidase （MPO） concentration was used to reflect the neutrophilic infiltration of heaptic tissue. And the injury severity of hepatic tissue was evaluated by the levels of serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） and the examinations of light microscopy and electron microscopy. Results The NF-kB level at 2 h （0.418 ±0.039） post-reperfusion was significantly higher in the I/R group. And it decreased gradually at 8 h （0. 308 ±0. 028） and 24 h （0. 240 ± 0. 032）.And the ICAM-1 level increased at 2 h （0. 367 ±0. 034） post-reperfusion, peaked at 8 h（0.451 ±0. 031）and began to decrease at 24 h （0. 293 ± 0. 025）. The changes of MPO, ALT and AST were similar to ICAM-1. The above-mentioned indicators at different time-points were lower in the P group than those of the I/R group （P 〈0. 05） , but higher than the S group （P 〈0.05）. The changes in ALT, MPO, NF-κB and ICAM-1 had a positive correlation （ P 〈 0.01 ） . Light and electron microscopy observations showed significant differences in different groups. Conclusion The rat hepatic ischemia-reperfusion increases the expressions of NF-kB and ICAM-1 and they are involved in hepatic ischemia-reperfusion injury. Salvia significantly reduces the expressions of NF-κB and ICAM-1 in ischemia-reperfusion hepatic tissue and it may effectively protect the injury of hepatic ischem

关 键 词：肝 再灌注损伤 NF—κB 胞间黏附分子1 丹参 

分 类 号：R285.5[医药卫生—中药学]