抗感颗粒抗甲型流感病毒的实验观察  

Study on the activity against influenza A virus with traditional Chinese medicine Kanggan granules

作　　者：刘冯欢[1] 李明远[1] 罗俊[1] 李婉宜[1] 王保宁[1] 杨远[1] 邝玉[1] 左斌[1] 马秀英 蒋忠华[1] 

机构地区：[1]四川大学华西基础医学与法医学院微生物学教研室,成都610041 [2]四川好医生攀西药业有限责任公司

出　　处：《中华医学杂志》2010年第26期1863-1865,共3页National Medical Journal of China

摘　　要：目的 观察抗感颗粒在鸡胚和BALB/c小鼠体内抗甲型流感病毒的作用.方法 （1）鸡胚实验：将甲型流感病毒鼠肺适应株FM1接种9日龄鸡胚,再将不同剂量的抗感颗粒注入鸡胚,以血凝效价为指标,观察抗感颗粒对流感病毒的作用,设立流感病毒对照组和利巴韦林片对照组,每组6个鸡胚.（2）小鼠实验：将BALB/c小鼠随机分为病毒感染模型组、正常对照组、利巴韦林片组及抗感颗粒低、中、高剂量组,每组20只小鼠.用流感病毒感染小鼠后灌胃给药,以实验小鼠的生存情况、死亡保护率及延长生命率等指标观察抗感颗粒的抗流感病毒作用.结果 在鸡胚实验中,抗感颗粒高、中剂量组均能抑制流感病毒复制并达到显效结果,病毒血凝效价比流感病毒对照组分别低了5个、3个稀释度.在小鼠实验中,抗感颗粒各剂量组均能减轻感染小鼠的发病症状,减少小鼠死亡,死亡保护率为35.0%～55.0%;生存时间延长,延长生命率为73.0%～88.9%、最低有效剂量为3.00g/kg.结论 抗感颗粒对甲型H1N1流感病毒的增殖有抑制作用,对流感病毒感染引起的小鼠死亡有保护作用.Objective To study the anti-influenza A virus effects of traditional Chinese medicine Kanggan granules in chicken embryo and BALB/c mice. Methods The influenza A virus （ H1N1, FM1） was used in the experiments. FM1 was cultured in chicken embryo and the anti-FM1 activity of Kanggan granules was evaluated through the post-medication hemagglutination titer of FM1. In animal test, 120 healthy BALB/c mice were randomly divided into six groups, normal control, virus control, ribavirin, highdosage, middle-dosage and low-dosage. The FM1 infection model was established by dripping FM1 into nasal cavity and then the appropriate treatments were prescribed. The effective anti-FMl indices of Kanggan granules included survival status, protective percentage of death and life elongation percentage of mice infected with FM1. Results The high and middle doses of Kanggan granules could inhibit the replication of FM1 remarkably in chicken embryo, and could reduced hemagglutination titer to 5 and 3 times. In animal experiments, all mice treated with Kanggan granules could improve the general status of infected mice, the protective percentages of death were 35. 0% to 55. 0% , the life elongation percentages were 73. 0% to 88. 9% and the minimal effective dose was 3. 00 g/kg. Conclusion Kanggan granules can inhibit the replication of influenza A virus and protect the mice infected with FM1.

关 键 词：流感病毒A型 动物 实验 抗感颗粒 H1N1 

分 类 号：R28[医药卫生—中药学]