奥替普拉在阻断细胞恶性转化时对某些基因表达的影响  

EFFECT OF SOME GENES EXPRESSION IN INHIBITION OF CELLUAR MALIGNANT TRANSFORMATION BY OLTIPRAZ

作　　者：柏华[1] 罗贤懋[1] 胡国刚[1] 魏慧娟[1] 李中骞[1] 

机构地区：[1]中国协和医科大学中国医学科学院肿瘤研究所

出　　处：《药学学报》1999年第3期232-234,共3页Acta Pharmaceutica Sinica

摘　　要：奥替普拉（ｏｌｔｉｐｒａｚ）来源于十字花科（Ｃｒｕｃｉｆｅｒａｓｅ）蔬菜，全称５（２吡嗪基）４甲１，２２羟基３硫酮，文献报道它为一种防癌抑癌剂［１］。我们用致癌素香烟凝聚物（ｃｉｇａｒｅｔｅｓｍｏｋｉｎｇｃｏｎｄｅｎｓａｔｅＣＳＣ）诱发...AIM: To investigate the effect of some gene expression in inhibition of celluar malignant transformation by oltipraz. METHODS: Human fetal lung (HFL) treated with cigarette smoking condensate (CSC) and / or oltipraz was extracted for DNA and transfected into Rat 1 cell. C Ha ras gene mutation of Rat 1 and transformated Rat 1 cell were analyzed by PCR RFLP. GST activity and GST π were examined by colorimetric determination and ELISA. RESULTS: Oltipraz can decrease GST activity and GST π protein content along with its inhibition of HFL carcinogenesis, but no obvious effect on mP53 gene expression. CONCLUSION: The effects of GST π and C Ha ras gene may be involved in inhibition of cellular malignant transformation by oltipraz.

关 键 词：抗癌药 奥替普拉 CST-π 基因表达 

分 类 号：R979.1[医药卫生—药品]