离子色谱法同时测定化妆品中的铵和6种烷基胺  

Simultaneous determination of ammonium and six alkylamines in cosmetics by ion chromatography

作　　者：钟志雄[1] 李攻科[2] 朱炳辉[1] 罗志彬[1] 吴西梅[1] 

机构地区：[1]广东省疾病预防控制中心,广东广州510300 [2]中山大学化学与化学工程学院,广东广州510275

出　　处：《色谱》2010年第7期702-707,共6页Chinese Journal of Chromatography

基　　金：广东省医学技术研究基金(No. A2007072)

摘　　要：建立同时测定化妆品中铵和6种烷基胺的离子色谱(IC)分析方法。优化了色谱条件和样品前处理方法,样品经100mmol/L乙酸-20%(v/v)乙腈溶液浸提,固相萃取(SPE)柱去除阴离子、中和氢离子后进样测定。考察了提取溶液的pH、有机溶剂和共存离子对测定结果的影响。分析方法的线性范围为0.3 ～15mg/L,检出限为2.1 ～7.9mg/kg,定量限为7 ～26mg/kg。采用建立的分析方法测定了清洗、柔肤、祛斑、防晒、烫发、染发和育发类化妆品的加标回收率的范围在80.2%～109.2%之间,相对标准偏差(RSD)的范围为0.5%～3.1%。方法选择性好,灵敏度高,抗干扰强,用于实际样品测定结果准确。A method for the simultaneous determination of ammonium and six alkylamines in cosmetic products by ion chromatography （IC） was developed.The sample pretreatment process and the separation resolution of chromatography were investigated.The samples were extracted by 100 mmol/L acetic acid-20%（v/v） acetonitrile solution at room temperature,and then solid phase extraction （SPE） column was used to eliminate the interferences.The influences of pH value,organic solvent and coexisted ions were investigated.The separation was carried out on IonPac CS17 （250 mm×4 mm）analytical column and IonPac CG17 （50 mm×4 mm）guard column using 1.5-15 mmol/L methanesulfonic acid and 0.5%-5%（v/v） acetonitrile gradient elution at a flow rate of 1.0 mL/min at 24 ℃,coupled with suppressed conductivity detector.Under the optimum conditions,a measurement could be completed less than 26 min.The linearity ranged from 0.3 to 15 mg/L,the detection limits and the quantification limits were in the ranges of 2.1-7.9 mg/kg and 7-26 mg/kg,respectively.The method was successfully employed for the determination of ammonium,methylamine,dimethylamine,trimethylamine,ethylamine,propylamine and butylamine in samples including cleaning,body lotion,skin-bleaching,sun block,marcel,hair dye and pilatory cosmetics with the recoveries of 80.2%-109.2% and the relative standard deviations （RSDs） of 0.5%-3.1%.The method offered high selectivity,sensitivity,and gave satisfactory results for real sample analysis.

关 键 词：离子色谱法 铵 烷基胺 化妆品 

分 类 号：O658[理学—分析化学]