辽宁省药用植物根际土壤木霉属真菌的多样性  

Diversity of Trichoderma spp.isolated from medicinal plant rhizosphere soils in Liaoning Province.

作　　者：潘争艳[1,2] 傅俊范[2] 马亮[3] 王慧[2] 

机构地区：[1]辽宁省农业科学院稻作研究所,辽宁沈阳110101 [2]沈阳农业大学植物保护学院,辽宁沈阳110161 [3]辽宁省农业科学院蚕业科学研究所,辽宁凤城118100

出　　处：《浙江大学学报（农业与生命科学版）》2010年第4期405-410,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家"十一五"科技支撑计划资助项目(2006BAI09B04-09)

摘　　要：对采集于辽宁省药用植物62份土壤样品的木霉属真菌进行分离,获得木霉属真菌78株,采用形态学方法从中鉴定出9种木霉属真菌,分别是哈茨木霉(Trichoderma harzianum)、绿色木霉(T.viride)、粗壮木霉(T.strigosum)、长枝木霉(T.longibrachiatum)、卷曲木霉(T.spirale)、钩状木霉(T.hamatum)、康氏木霉(T.koningii)、顶孢木霉(T.fertile)和多孢木霉(T.polysporum).其中哈茨木霉分离到34株,占分离到木霉菌总数的43.6%.采用对峙培养法,共筛选出对5种靶标菌抑制率大于70%,对靶标菌菌丝抑制作用明显的菌株4株,其中哈茨木霉2株,绿色木霉1株,钩状木霉1株.Seventy-eight isolates of Trichoderma spp.were isolated from 62 soil samples of medicinal plant rhizosphere in Liaoning Province.Morphological identification showed that they belonged to 9 species of Trichoderma,namely Trichoderma harzianum,T.viride,T.strigosum,T.longibrachiatum,T.spirale,T.hamatum,T.koningii,T.fertile and T.polysporum.Isolated 34 strains of T.harzianum accounted for 43.6% of the total isolated Trichoderma.Four Trichoderma strains including two strains of T.harzianum,one strain of T.viride and one strain of T.hamatum,which could inhibit the mycelial growth of pathogenic bacteria,were screened out by face-to-face-culturing on PDA media,and their inhibitory efficiencies against five kinds of pathogens reached more than 70%.

关 键 词：药用植物 木霉菌 多样性 拮抗 

分 类 号：S154.3[农业科学—土壤学] Q939[农业科学—农业基础科学]