硫酸锌浸种对水稻幼苗生长和细胞保护酶活性的影响  

Effects of seed soaking with zinc sulfate on growth and cell protective enzyme activity of rice seedlings.

作　　者：孟杰[1] 王人民[1] 万吉丽[1] 付力成[1] 

机构地区：[1]浙江大学农业与生物技术学院作物科学研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2010年第4期411-418,共8页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30471021);国家杰出青年科学家基金资助项目(39925024)

摘　　要：以IR8192(Indica)和BY(Japonica)为材料,设置不同的硫酸锌浓度(0,0.0005%,0.001%,0.005%,0.01%,0.05%,0.10%,0.15%和0.25%),研究硫酸锌溶液浸种对水稻幼苗生长及其生理特性的影响.结果表明:适量的硫酸锌溶液浸种促进了水稻幼苗的生长和干物质量的积累,增加了水稻叶片中叶绿素a、b、a+b的含量,增强了超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,降低了丙二醛(MDA)含量,增加了可溶性蛋白和可溶性糖的含量.不同水稻品种最适宜的浸种浓度不同,IR8192以0.001%浓度的硫酸锌溶液处理效果最好,而0.005%浓度的硫酸锌溶液浸种对BY作用效果最佳,对IR8192则产生了毒害作用.随着浸种浓度的继续增加,超过植株的耐受范围,幼苗生长开始受到抑制.表明适量的硫酸锌溶液浸种可以促进水稻幼苗的生长,提高水稻幼苗生长过程中的抗氧化物酶活性,减缓脂质过氧化作用,增强物质代谢水平,但超过植物的锌耐受范围则会产生重金属毒害作用.The effect of seed soaking with zinc sulfate on growth and physiological characteristics of rice seedlings was studied using seeds of IR8192 and BY typed rice as subjects treated with different concentrations of zinc sulfate（0,0.0005%,0.001%,0.005%,0.01%,0.05%,0.10%,0.15%,0.25%）.The results showed that rice seeds treated with seed soaking in appropriate zinc concentration promoted plant growth and accumulation of dry matter,increased the amount of chlorophyll a,b and a＋b in leaves,enhanced the superoxide dismutase（SOD）,peroxidase（POD）,catalase（CAT） activity,reduced malondialdehyde（MDA） content and increased soluble protein and soluble sugar content.However,the optimal zinc concentrations of seed soaking were different as to different types of rice.IR8192 typed rice treated with 0.001% concentration of zinc sulfate had the best treatment effect,while the optimal concentration of BY typed rice was 0.005% which resulted in toxic effect on IR8192.With the zinc concentration of seed soaking going on increasing until it exceeded plant tolerance,growth of rice seedlings would begin to be inhibited.This experiment indicates that seed soaking in appropriate concentration of zinc sulfate can promote growth of rice seedlings,enhance the level of antioxidant enzyme in the course of growth,slow down the role of lipid peroxidation and enhance the level of material metabolism,yet zinc concentration exceeding plant tolerance will result in toxic effect on rice seedlings.

关 键 词：硫酸锌 水稻幼苗 生理特性 

分 类 号：S330.2[农业科学—作物遗传育种]