瑞香素及其衍生物对2型糖尿病大鼠微循环影响的实验研究  

Effect to microcirculation induced by daphnetin and its derivative on type 2 diabetic rats

作　　者：李红[1] 李伟平 柳溪[1] 王吉平 杨世杰[1] 

机构地区：[1]吉林大学白求恩医学院药理学系,吉林长春130021 [2]吉林省西点药业科技发展股份有限公司,吉林长春130033

出　　处：《吉林医学》2010年第19期2981-2983,共3页Jilin Medical Journal

摘　　要：目的:研究瑞香素及其衍生物对2型糖尿病微循环的影响。方法:采用高脂饮食结合小剂量应用链脲佐菌素(30 mg/kg)建立实验性2型糖尿病(2型DM)大鼠模型。通过对大鼠血糖值、体外血栓及肠系膜微动、静脉血流速度、血管管径的检测,镜下观察肠系膜微血管血流运动的状态,观察瑞香素及其衍生物对微循环影响。结果:与模型组比较,消渴丸(3.0 g/kg)组、联合给药(3.5 g/kg)组均能降低血糖(P<0.05,P<0.01);而瑞香素(0.5 g/kg)组、瑞香素衍生物(1.0 g/kg、0.5 g/kg、0.25 g/kg)组对血糖有降低趋势,但无统计学意义(P>0.05);瑞香素(0.5 g/kg)、瑞香素衍生物(1.0 g/kg、0.5 g/kg)、消渴丸(3.0 g/kg)及联合给药(3.5 g/kg)均能抑制血栓形成长度明显缩短(P<0.05,P<0.01)、血栓湿重减轻(P<0.05,P<0.001)及血流速度明显加快(P<0.05、P<0.01)、微血管管径均明显增大(P<0.05);糖尿病模型组大鼠微动、静脉可观察到线粒流;各给药组大鼠微动、静脉的流速多为线流,少数可见线粒流。结论瑞香素及其衍生物可明显改善2型DM大鼠微循环障碍。Objective To study the effect to microcirculation induced by daphnetin and its derivative on type 2 diabetic rats.Method To establish experimental type 2 diabetes mellitus（type 2 DM） rats by high-fat diet combined with small doses of streptozotocin（30 mg/kg）.To observe the effect of daphnetin and its derivatives on the microcirculation through the detection of the blood glucose values,thrombosis in vitro and mesenteric artery and vein blood flow velocity,vascular diameter,the observation of the movement state of mesenteric microvascular blood flow under the microscope.Results Compared with model group,Xiaoke pill（3.0 g/kg） group,co-administration（3.5 g/kg） group can lower blood glucose（P〈0.05,P〈0.01）;while daphnetin（0.5 g/kg） group,daphnetin derivative （1.0 g/kg,0.5 g/kg,0.25 g/kg） group had the trend to lower blood glucose,but showed no statistical significance（P〈0.05）;daphnetin（0.5 g/kg）,daphnetin derivatives（1.0 g/kg,0.5 g/kg）,Xiaoke pill（3.0 g/kg） and co-administration（3.5 g/kg） inhibiting thrombosis length was significantly shorter（P〈0.05,P〈0.01）,thrombus wet weight decreased（P〈0.05,P〈0.001） and blood faster than it（P〈0.05,P〈0.01）,microvessel diameter significantly increased（P〈0.05）;diabetic model rats micro-arteries and veins can be observed line particle flow;micro drug-treated rats and venous flow velocity over the line,a few can be seen line grain flow.Conclusion Daphnetin and its derivatives can improve the microcirculation in type 2 DM rats.

关 键 词：瑞香素 瑞香素衍生物 2型糖尿病 微循环 

分 类 号：R285.5[医药卫生—中药学]