茭白黑粉菌及茭白植株中DNA的提取方法  

Efficient Method for DNA Isolation from Ustilago esculenta and Zizania latifolia

作　　者：徐蝉[1] 张敬泽[1] 刘娜[1] 徐晓峰[1] 黄建中[1] 郭得平[1] 

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058

出　　处：《长江蔬菜》2010年第14期32-35,共4页Journal of Changjiang Vegetables

基　　金：国家公益性行业(农业)科研专项经费项目(200903017-03)

摘　　要：研究了茭白黑粉菌及茭白植株中基因组DNA的高效提取方法。采用改进的CTAB(十六烷基三甲基溴化铵)法提取DNA,根据琼脂糖凝胶电泳检测、A260/A280的测定和PCR扩增结果表明,用改进的CTAB法提取茭白黑粉菌及茭白植株基因组DNA,所得DNA无论是纯度还是完整性都比较好,适合于酶切和PCR扩增,可以用于分子生物学研究。An efficient method was developed for rapid isolation of total genomic DNA from Ustilago esculenta and Zizania latifolia. The results of agarose gel electrophoresis showed that the A260/A280 ratio and PCR amplification indicated that the isolated DNA was suitable for restriction endonuclease digestion and polymerase chain reaction （PCR） amplification,and was used for molecular bilolgy research.

关 键 词：茭白 茭白黑粉菌 DNA CTAB法 

分 类 号：S645.2[农业科学—蔬菜学]