细胞色素c在纳米Mn_3O_4修饰玻碳电极上的直接电化学  

Direct electrochemistry of cytochrome c on nano-Mn_3O_4 modified glassy carbon electrode

作　　者：张瑞娟[1] 尹起范[1,2] 徐继明[2] 朱玉兰[2] 马奎蓉[2] 

机构地区：[1]延边大学理学院,吉林延吉133002 [2]淮阴师范学院化学化工学院江苏省低维材料化学重点建设实验室,江苏淮安223300

出　　处：《化学试剂》2010年第7期577-579,651,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(20671038);江苏省教育厅自然科学基金项目(06KJB150011);江苏省低维材料化学重点建设实验室开放课题资助项目(JSKC09068)

摘　　要：采用循环伏安法探讨了细胞色素c(Cyt c)在纳米Mn3O4修饰玻碳电极表面的直接电化学行为。实验结果表明,Cyt c在纳米Mn3O4修饰玻碳电极上呈现一对峰形较好且准可逆的氧化还原峰,其式电位(E0)为56 mV,峰电流与扫描速度呈线性关系,表明电极过程是一个表面控制过程,电化学反应效率常数(ks)为4.25 s-1,固定在Mn3O4上的Cyt c能促进过氧化氢的催化还原,其实验结果可为新型传感器的研制提供依据。The direct electrochemistry of cytochrome c（Cyt c） immobilized on Mn3O4 nanoparticle-modified glassy carbon electrode was investigated by cyclic voltammetry（CV）.The results showed that a pair of well-defined quasi-reversible redox peaks of Cyt c was obtained at Nafion/Cyt c/Mn3O4/GCE based enzyme electrode by the direct electron transfer between the protein and the electrode.The peak current was linearly dependent on the scan rate,indicating that the direct electrochemistry of Cyt c in that case was a surface-controlled process.The Cyt c immobilized on Mn3O4 can promote catalytic reduction of hydrogen peroxide.The experimental results can provide a basis for a new type of sensor.

关 键 词：细胞色素C 纳米Mn3O4 直接电化学 

分 类 号：O657.1[理学—分析化学]