冬虫夏草水提取物对慢性低氧大鼠肺动脉平滑肌细胞增殖及癌基因c-fos、c-jun表达的影响  

Effects of water extract from Cordyceps sinensis on hypoxia-induced proliferation and oncogene expression of rat pulmonary artery smooth muscle cells

作　　者：高宝安[1] 陈世雄[1] 邓红艳[1] 向光明[1] 黄骥[1] 崔向军[1] 杨俊[1] 

机构地区：[1]三峡大学第一临床医学院/湖北省宜昌市中心人民医院,443003

出　　处：《重庆医学》2010年第15期1957-1959,1962,共4页Chongqing medicine

基　　金：湖北省卫生厅青年人才基金资助项目(QJX2008-21);湖北省宜昌市医疗卫生科技计划资助项目(A2008302-07)

摘　　要：目的探讨冬虫夏草水提取物对慢性低氧大鼠肺动脉平滑肌细胞(PASMCs)增殖的影响及其可能的作用机制。方法用MTT法测定不同浓度冬虫夏草水提取物对慢性低氧大鼠PASMCs增殖的影响,用流式细胞仪分析冬虫夏草水提取物对慢性低氧大鼠PASMCs细胞周期的影响,用免疫细胞化学染色法测定冬虫夏草水提取物对慢性低氧大鼠PASMCs增殖细胞核抗原(PCNA)和癌基因c-fos、c-jun表达的影响。结果低氧对照组大鼠PASMCs吸光度(A)值明显高于常氧对照组,差异有统计学意义(P<0.01),1、10、100mg/mL冬虫夏草组A值呈明显下降趋势,差异均有统计学意义(P<0.05);低氧对照组S+G2M期细胞比例明显高于常氧对照组,差异有统计学意义(P<0.01),冬虫夏草组S+G2M期细胞比例明显低于低氧对照组,但高于常氧对照组,差异均有统计学意义(P<0.01);冬虫夏草组PCNA阳性表达率明显低于低氧对照组,但高于常氧对照组,差异均有统计学意义(P<0.01);冬虫夏草组细胞c-fos和c-jun蛋白阳性染色平均积分光密度值(AIOD)明显低于低氧对照组,但高于常氧对照组,差异均有统计学意义(P<0.01)。结论冬虫夏草水提取物呈浓度依赖性抑制慢性低氧导致的大鼠PASMCs增殖,其抑制作用可能是通过减少细胞内c-fos、c-jun基因和PCNA的表达、降低S期和G2/M期细胞比例来实现的。Objective To investigate effects of water extract from herb Cordyceps sinensis on hypoxia-induced proliferation of rat pulmonary artery smooth muscle cells（PASMCs） and its mechanism in vitro. Methods Cell growth inhibitions of different con- centrations of water extract from Cordyceps sinensis on rat PASMCs were analyzed by MTT assay;Cell cycles of rat PASMCs trea- ted with water extract from Cordyceps sinensis were analyzed by cytofluorometry;Expressions of proliferating cell nuclear antigen （PCNA） ,e-fos,and c-jun of rat PASMCs were measured by immunocytochemistry. Results MTT assay showed,relative ratio A of ahsorbance of rat PASMCs in chronic hypoxia group was higher significantly than ratio A in normoxia group（P〈0.01） ;Ratio A of different Cordyceps sinensis group was decreased when the concentration of Cordyceps sinensis was increased（all P〈0.05）. Percentage of cells in the S＋G2/M phase of chronic hypoxia group was higher significantly than that of normoxia group（P〈0.01）; Percentage of cells in the S＋G2/M phase of Cordyceps sinensis group was higher significantly than that of normoxia group, but lower remarkably than that of hypoxia group（all P〈0. 05）. Positive percentage of PCNA in Cordyceps sinensis group was lower significantly than that in hypoxia group, but higher remarkably than that in normoxia group（ all P〈0.01）. Average integrated optical density（AlOD） of c-los and c-jun proteins in Cordyceps sinensis group were lower significantly than AIOD in hypoxia group, but higher remarkably than AIOD in normoxia group（all P〈0.01）. Conclusion Water extract from Cordyceps sinensis can inhibit rat PASMCs hypoxia-induced proliferation in a concentration-dependant manner; The mechanism could be to suppress expression of PCNA, c-fos,e-jun,and decrease percentage of cell in S and G2/M phase.

关 键 词：冬虫夏草水提取物 大鼠肺动脉平滑肌细胞 低氧 增殖 癌基因 

分 类 号：R285.5[医药卫生—中药学]