参麦注射液对心肌缺血再灌注损伤大鼠PECAM-1表达的影响  

Effect of Shenmai injection on the changes of Platelet endothedial cell adhesion molecule-1(PECAM-1) on myocardial ischemia/reperfusion injury in rats

作　　者：刁玉晶[1] 赵明[2] 蒋鹏[2] 尤海燕[3] 马鹏 

机构地区：[1]江苏大学临床医学院,镇江212001 [2]江苏大学附属医院麻醉科,镇江212001 [3]江苏大学附属医院中心实验室,镇江212001

出　　处：《重庆医学》2010年第15期1973-1974,共2页Chongqing medicine

摘　　要：目的研究参麦注射液对心肌缺血/再灌注损伤大鼠血小板内皮细胞黏附分子-1(PECAM-1)表达的影响。方法取30只SD大鼠随机分为假手术组(1组)、缺血再灌注组(2组)、参麦组(3组)。开胸结扎冠状动脉,造成心肌缺血,30min后放松再灌注90min,建立心肌缺血再灌注模型。3组每天注射参麦注射液10mL/kg,连续3d,1、2组注射等量生理盐水。用流式细胞术检测血中PECAM-1蛋白表达。结果与1组比较,2组血中中性粒细胞和淋巴细胞PECAM-1/CD31蛋白表达明显升高。3组与2组比较,血中PECAM-1/CD31蛋白表达降低。结论参麦注射液对大鼠心肌缺血再灌注损伤有一定保护作用。Objective To investigate the effects of Shenmai injection on the changes of Platelet endothedial cell adhesion molecule I（PECAM-1） after myocardial ischemia/reperfusion（MIR）in rats. Methods Thirty SD rats were randomly divided into three groups： 1 group（operation group）,2group（isehemia-reperfusion without any intervention）, 3 group（administered with Shenmai in- jection ） ,then 10 mL/kg Shenmai injection were given in Shenmai group and the 0.9%NaC1 l0 mL/kg were given in t group and 2 group for three days. After thoraeotomy and ligation,the rats were injured by ischemia and relaxed 30 minutes later, then reperfusioned for 90 minutes,the myocardial ischemia-reperfusion models in rats were established during the experiment. The PECAM-1 protein expressions were evaluated by FCM method. Results Compared with 1 group,the expressions of PECAM-1/CD31 in neutrophilic granulocytes and lymphocytes from blood in 2 group were increased significantly. Compared with 2 group,the expressions of PECAM-1/CD31 fr6m blood in 3 group were decreased. Conclusion Shenmai injection may protect myocardium isehemia/reperfusion injury.

关 键 词：缺血/再灌注损伤 血小板内皮细胞黏附分子-1 参麦注射液 

分 类 号：R285[医药卫生—中药学]