转铁蛋白基因在三种淡色库蚊体内表达量的比较分析  

Comparative analysis of expression level of transferrin gene in 3 strains of Culex pipiens pallens

作　　者：谭文彬[1] 王霄[2] 魏继波[3] 程鹏[3] 全芯[2] 高红刚[2] 

机构地区：[1]济宁医学院临床技能中心,山东济宁272000 [2]济宁医学院病原生物学教研室,山东济宁272013 [3]山东省寄生虫病防治研究所,山东济宁272033

出　　处：《中国热带医学》2010年第9期1050-1051,共2页China Tropical Medicine

基　　金：山东省教育厅课题(J09LF04);济宁市科委课题(2009年第18项)

摘　　要：目的进行不同品系蚊转铁蛋白基因表达量的分析。方法据二维电泳获得肽段序列设计引物扩增基因片段,荧光定量PCR检测在淡色库蚊抗性品系品系、敏感品系、现场采集品系蚊体内的表达水平差异。结果成功扩增淡色库蚊转铁蛋白基因片段,荧光定量PCR表明转铁蛋白基因在抗性品系蚊体内的表达量是敏感品系的13.52倍,现场品系是敏感品系的7.48倍。结论淡色库蚊转铁蛋白基因可做为新的抗性检测及治理基因靶标。Aim To analyze ecpression level of transferrin gene in different stains of Culex pipiens pallens.Methods Primers were designed on the basis of two-dimensional gel electrophoresis peptide fragment sequence,fluorescence quantitative PCR was used to detect the expression level among resistant strain,sensitive strain and local strain of Culex pipiens pallens.Results The transferrin gene of Culex pipiens pallens were cloned successfully.FQ-PCR proved the transferrin gene expression level in resistant strain was 13.52 times higher than that of sensitive strain,while the expression level in local strain was 7.48 times higher than that of sensitive strain.Conclusion The transferrin gene of Culex pipiens pallens can be used as a new gene target for resistance detection and treatment.

关 键 词：淡色库蚊 转铁蛋白 荧光定量PCR 

分 类 号：R384.1[医药卫生—医学寄生虫学]