异黄酮苷元对快速老化小鼠神经保护的机制研究(英文)  

Neuro-protective Mechanism of Isoflavones on Senescence-accelerated Mice

作　　者：杨红[1,2] 金桂芳[2] 任冬冬[2] 罗思靖[2] 周天鸿[1] 

机构地区：[1]暨南大学生命科学技术学院,广州510632 [2]广东药学院基础学院,广州510006

出　　处：《中国天然药物》2010年第4期280-284,共5页

基　　金：supported by Guangdong Technology Bureau (Nos. 2004B30101001 and 2009B030801343);research and development fund from Guangdong Chinese Medicine Administration (No.103079)~~

摘　　要：目的:探讨异黄酮苷元对快速老化小鼠神经保护的作用机制。方法:以不同剂量异黄酮苷元灌胃治疗SAM-P/8快速老化小鼠;检测小鼠皮层乙酰胆碱酯酶(AchE)活性;检测小鼠血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)浓度;荧光法测定海马组织β-分泌酶活力;显微镜观察海马组织神经元细胞的变化。结果:治疗组小鼠皮层AchE活性、血清SOD和GSH-Px的活力明显高于模型组(P<0.05);MDA水平、海马组织β-分泌酶活力均低于模型组(P<0.05),海马神经元数量和细胞形态明显改善。结论:异黄酮苷元通过调节AchE活性,间接提高胆碱能神经递质ACh含量,改善中枢胆碱能功能,增强学习记忆能力;且具有强抗氧化活性,并通过降低海马β-分泌酶活性,减少不溶性Aβ碎片的产生和沉积,减轻其导致的神经毒性和神经元细胞的损伤,有效发挥神经保护作用。AIM：To investigate the neuro-protective mechanism of isoflavones,using senescence-accelerated SAM-P/8 mice.METHODS：Various dosages of isoflavones were intragastrically administered to SAM-P/8 senescence-accelerated mice.The cortex acetylcholinesterase（AchE） activity,serum superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） activities were examined.In addition,the concentration of malondialdehyde（MDA） was examined.β-secretase activity in the hippocampus tissue was determined by fluorometry.Changes of the hippocampal neurons were observed under a light microscope.RESULTS：Mice treated with isoflavones performed significantly better in the cognitive test than the no-treatment control group（P 0.05）.The cortex AchE activity,serum SOD and GSH-Px activities were notably higher than those of the untreated mouse（P 0.05）.MDA concentration and the β-secretase activity in the hippocampal tissue were both lower than those in no-treatment control（P 0.05）.The numbers of hippocampal neurons of isoflavones treatment mice were increased and the cellular morphology was significantly improved.CONCLUSION：Isoflavones can indirectly increase the concentration of the cholinergic neural transmitter Ach through regulation of AchE.Through reduction of hippocampal β-secretase activity,the strong anti-oxidative activity of isoflavones can decrease the formation and deposition of insoluable β-amyloid（Aβ） debris,relieve the resulting toxicity and damage to neurons,and thereby effectively protect the nervous system.

关 键 词：异黄酮苷元 快速老化小鼠 乙酰胆碱酯酶 Β-分泌酶 

分 类 号：R965[医药卫生—药理学]