外源血小板生成素基因体内导入对循环免疫因子水平的影响  

Effect of thrombopoietin gene delivery on the concentration of serum cytokines in mice

作　　者：赵建增 刘丽[1] 梅英杰[1] 

机构地区：[1]空军总医院空军临床分子生物学研究中心

出　　处：《中国免疫学杂志》1999年第3期103-105,共3页Chinese Journal of Immunology

摘　　要：目的：研究外源ＴＰＯ基因由质粒载体导入后，小鼠体内一些细胞因子的变化规律。方法：用脂质体将表达人ＴＰＯ的真核表达质粒ｐｃＤＮＡ３／ｈＴＰＯ包裹后，注射到Ｂａｌｂ／ｃ鼠的后肢肌肉内，用ＥＬＩＳＡ法检测小鼠血液中多种细胞因子的水平。结果：在ＴＰＯｃＤＮＡ转移到体内后，血液中ＩＦＮγ、ＴＮＦα和ＩＬ２迅速升高。其中，ＩＦＮγ与血小板计数呈明显负相关，并且在血小板计数回落后，保持在对照水平的９～１２倍；ＴＮＦα仅在第１周内与之成正相关，但可达对照水平的１２０倍；ＩＬ２升高幅度较小，与ＴＰＯ水平相一致。血液中Ⅱ型组织相容性抗原分子也呈升高趋势，与ＩＬ２的变化类似；而Ⅰ型分子与ＴＮＦα的变化相似。结论：ＴＰＯ在多方面刺激了机体免疫机能，而免疫因子如ＩＦＮγ在血小板生成过程中起重要的调节作用。Objective:To investigate the effect of TPO on the expression of cytokines.Methods:The liposome coated DNA of plasmid pcDNA3 carring human thrombopoietin(TPO) cDNA was intramuscularly administered into Balb/c mice.The serum cytokine levels were determined using ELISA.Results:Soon after the gene delivery,serum IFN γ,TNF α and IL 2 abruptly increased.IFN γ level was negatively associated with platelet count(PLTC) in the stage of PLTC declination,it reached to 9～12 folds more than that of the controls.Although TNF α level had reached 120 folds more than that of the control in the first week,positively associated with the PLTC. The increase of IL 2 was not significant. Furthermore,class Ⅱ molecules of major histocompatibility were also increased, while class I molecules showed no significant changes except the first week.Conclusion:TPO might participate in immunoregulation.And IFN γ might play key role in down regulation of the thrombopoiesis.

关 键 词：血小板生成素 免疫调节 IFN-Γ TNF-α IL-2 

分 类 号：Q511[生物学—生物化学] R392.1[医药卫生—免疫学]