系统性红斑狼疮患者肿瘤坏死因子与HLA-DR基因关联的研究  

Study on the association between HLA DR and Tumor Necrosis Factor genes in the patients with Systemic Lupus Erythematosus

作　　者：彭学标[1,2,3] 费虹明[1,2,3] 徐文严 

机构地区：[1]第一军医大学南方医院皮肤科 [2]上海第二医科大学生物学教研室 [3]中国医学科学院皮肤病研究所

出　　处：《中国免疫学杂志》1999年第3期125-126,共2页Chinese Journal of Immunology

摘　　要：目的：探讨系统性红斑狼疮（ＳＬＥ）的发病机理和遗传基础。方法：用聚合酶链反应结合顺序特异的寡核苷酸（ＰＣＲ／ＳＳＯ）探针杂交方法对７１例汉族ＳＬＥ患者和６９例正常对照组ＨＬＡＤＲ亚区作ＤＮＡ分型，同时用双抗体夹心ＥＬＩＳＡ法检测血清中ＴＮＦα水平。结果：发现汉族ＳＬＥ患者中ＤＲ２频率显著高于正常对照组，而ＤＲ４频率则明显低于正常对照组，活动期ＳＬＥ患者血清ＴＮＦα水平明显高于非活动期ＳＬＥ患者和正常对照组，而非活动期ＳＬＥ患者和正常对照组血清ＴＮＦα水平无明显差异。ＤＲ２阳性的ＳＬＥ患者ＴＮＦα水平低，而ＤＲ４阳性的ＳＬＥ患者ＴＮＦα水平则较高。Objective:Study the pathogenesis and the genetic background of Systemic Lupus Erythematosus(SLE).Methods:To use polymerase chain reaction and sequence specific oligoncleotide(PCR/SSO) probe hybridization for typing HLA DR subregion in 71 cases of patients with SLE of Han nationality and 69 cases of matched controls.At the same time,used ELISA of sandwich with two antibodies to examine Tumor Necrosis Factor α(TNFα)in sera.Results:DR2 gene occured more frequent in patients than that of in controls,whereas DR4 gene occured less frequent in patients,compared with that of controls.TNFα level was much higher in the serum of SLE patients in the active stage than that of the patients and of that in the inactive stage and controls,but there was no difference between the latter.The level of TNFα was low in the patients with DR2 gene,but high in the with DR4 gene.Conclusion:Certain association of HLA DR and TNF genes might be related in the pathogenesis of SLE.

关 键 词：系统性 红斑狼疮 肿瘤坏死因子 HLA-DR 

分 类 号：R593.241[医药卫生—内科学]