人胎儿卵巢冷冻保存后两种复苏方法的比较研究  

A Comparative Study on The Two Thawing Methods of Cryopreserved Human Fetal Ovary

作　　者：谭丽[1] 郑英[1] 黄冬梅[1] 

机构地区：[1]河南医科大学第二附属医院

出　　处：《中国实用妇科与产科杂志》1999年第3期155-156,共2页Chinese Journal of Practical Gynecology and Obstetrics

摘　　要：目的探索卵巢移植中的移植物（胎儿卵巢）的体外保存方法。方法将１６例２０～２８周的胎儿卵巢置于含１０％二甲基亚砜（ＤＭＳＯ）的培养液中，采用三步冷冻法投入液氮保存。复温时采用４０℃水浴快速复温和室温下慢复温，然后进行组织培养观察卵泡发育和雌二醇（Ｅ２）分泌情况。结果胎儿卵巢复温组培养，其卵泡能进一步发育并产生Ｅ２；快复温组卵泡组织形态良好，并能维持良好的内分泌功能；慢复温组卵巢上皮破裂，Ｅ２分泌量明显减少。结论胎儿卵巢冷冻保存后，快速复温能更好地维持卵泡形态发育和内分泌功能。Objective\ To investigate the in vitro preservative method of donor grafts(fetal ovary) in clinical fetal ovary transplantation. Methods\ 16 human fetal ovaries of 20 to 28 weeks in the medium including 10% (v/v) DMSO in cryopreserved tubes were put into liquid nitrogen by 'three-step cooling procedure'. These cryopreserved ovaries were recovered with two thawing procedures-fast thaw in 40℃ water bath and slow thaw in room temperature. Thawed fetal ovaries were cultured to observe the follicular development and estradiol (E 2) secretion. Results\ The follicles in frozen-thawed fetal ovaries could develop to further maturity and secret estradiol after culturing in vitro. The ovaries in fast thaw group could maintain good follicular morphology and secreory function. But in the slow thaw group, ovary epithelium was completely disrupted and the amount of estradiol in medium reduced significantly. Conclusions\ To the cryopreserved human fetal ovaries, the fast-thaw method could maintain follicular development and secretory function much better than the slow-thaw method.

关 键 词：胎儿卵巢 冷冻保存 复苏 卵巢移植 

分 类 号：R713.6[医药卫生—妇产科学] R318.52[医药卫生—临床医学]