蝎毒抗癌多肽对4种人肿瘤细胞系的作用  

Effect of antineoplastic polypeptide from Buthus martensii venom on four kinds of human tumor cell lines

作　　者：董伟华[1] 孔天翰[1] 韩雪飞[1] 王成裕[1] 雷留根[1] 魏玲 郑湘豫[1] 

机构地区：[1]河南医科大学生物毒素中心

出　　处：《河南医科大学学报》1999年第1期52-55,共4页Journal of Henan Medical University

基　　金：河南省"九五"科技攻关项目

摘　　要：目的：观察蝎毒抗癌多肽（ａｎｔｉｎｅｏｐｌａｓｔｉｃｐｏｌｙｐｅｐｔｉｄｅｆｒｏｍＢｕｔｈｕｓＭａｔｅｎｓｉｖｅｎｏｍ，ＡＰＢＭＶ）对人肿瘤细胞生长的抑制作用。方法：采用噻唑蓝（ＭＴＴ）法、台盼蓝拒染法和集落形成观察为指标，丝裂霉素Ｃ（ＭＭＣ）为阳性对照药品，以ＡＰＢＭＶ４ｍｇ／Ｌ，８ｍｇ／Ｌ，１２ｍｇ／Ｌ和１６ｍｇ／Ｌ处理４种人肿瘤细胞（人早幼粒白血病细胞株ＨＬ６０、人肝细胞癌细胞株ＳＭＭＣ７７２１、人低分化鼻咽上皮癌细胞株ＣＮＥ２Ｚ和人胃癌细胞株ＭＧＣ８０３），选用不同的作用时间点，分别观察ＡＰＢＭＶ对上述细胞的影响。结果：ＡＰＢＭＶ对ＨＬ６０、ＳＭＭＣ７７２１、ＣＮＥ２Ｚ和ＭＧＣ８０３细胞均有显著的细胞毒性作用，ＨＬ６０和ＳＭＭＣ７７２１比后两者显示出更好的量效关系（ｒ＝０．９８２８，ｒ＝０．９８６３，Ｐ＝０．０２）。ＡＰＢＭＶ处理ＨＬ６０和ＳＭＭＣ７７２１细胞２４ｈ，４８ｈ和９６ｈ后，两种细胞的生长均受到抑制（Ｐ＜０．０５，Ｐ＜０．０１），并有显著的剂量效应关系；处理ＳＭＭＣ７７２１细胞２４ｈ，４８ｈ和９６ｈ时ＡＰＢＭＶ的ＩＣ５０分别是１５．８７ｍｇ／Ｌ、１３．０５ｍｇ／?Aim:To observe the inhibitory effects of antineoplastic polypeptide from Buthus martensii venom(APBMV) on human tumor celllines.Methods:Using the MTT colorimetric method, trypan blue exclusion and the colony formation as the indexes,the mitomycin C(MMC) as the positive control chemical, four kinds of human tumor cell lines HL 60,SMMC 7721,CNE 2Z and MGC 803 were treated with APBMV at different final concentrations of 4 mg/L,8 mg/L,12 mg/L and 16 mg/L The effects of APBMV on the cells mentioned above were observed at different intervals after the cells were exposured to APBMV.ResultsConclusion: APBMV possesses distinctly cytotoxicity and inhibitory growth effect on 4 kinds of human tumor cells,HL 60,SMMC 7721,CNE 2Z and MGC 803 cells.

关 键 词：蝎毒 细胞株 抗癌药 APBMV 

分 类 号：R979.1[医药卫生—药品]