舒喘灵对缺氧大鼠肺动脉高压和肺组织肾上腺素能β_2受体mRNA表达的影响  

Effect of salbutamol on pulmonary arterial pressure and lung β 2 adrenoceptor mRNA expresion in hypoxic rat

作　　者：丁耀贤[1] 王晓芳[1] 薛全福[1] 王立荣[1] 斯琴[1] 

机构地区：[1]中国医学科学院基础医学研究所中国协和医科大学基础医学院病理生理研究室

出　　处：《中国病理生理杂志》1999年第3期193-196,共4页Chinese Journal of Pathophysiology

基　　金：国家八五攻关项目

摘　　要：目的：研究β２激动剂舒喘灵对缺氧大鼠肺动脉高压及肺β２受体ｍＲＮＡ的作用。方法：常压缺氧４周，同时用舒喘灵灌胃（５ｍｇ·ｋｇ－１·ｄ－１），用肺动脉插管测压，用ＲＴ－ＰＣＲ术测肺β２受体ｍＲＮＡ。结果：缺氧同时给舒喘灵可减轻缺氧大鼠肺动脉高压、右心室压及右心室肥厚，同时使降低的β２受体ｍＲＮＡ回升到对照水平，动脉血氧分压不受影响，对体动脉压无明显作用。结论：舒喘灵能防止缺氧引起的肺动脉高压和肺肾上腺素能β２受体ｍＲＮＡ水平的降低。AIM:Effect of β 2 agonist salbutamol on pulmonary arterial pressure and lung β 2 adrenoceptor mRNA was investigated.METHOD:Rats underwent isobaric hypoxia for 4 weeks and salbutamol was poured into stomach(5mg·kg -1 ·d -1 )at some time.Pulmonary arterial pressure was measured.Weights of right ventricle(RV),left ventricle and septum(LV+S) were measured and RV/(LV+S) ratio was calculated.Lung β 2 adrenoceptor was measured by RT-PCR.RESULTS:Salbutamol could alleviate pulmonary arterial pressure,right ventricular pressure and right heart hypertrophy induced by hypoxia in rat.The decreased β 2 adrenoceptor mRNA recovered to normal control level at some time.No significant effect was found on arterial blood oxygen partial pressure and blood pressure.CONCLUSION:Salbutamol can alleviate pulmonary arterial pressure and right ventricular hypertrophy and the decrease of lung β 2 adenoceptor mRNA incuced by hypoxia in rat.

关 键 词：肺动脉高压 缺氧 舒喘灵 肾上腺素能 Β2受体 

分 类 号：R543.2[医药卫生—心血管疾病] R594.306[医药卫生—内科学]