肺动脉与冠状动脉内皮环氧合酶及TXA_2合酶基因表达的差异  

Expression of cyclooxygenase and thromboxane synthase genes in pulmonary and coronary arterial endothelial cells

作　　者：于江洲[1] 王迪浔[1] 

机构地区：[1]同济医科大学病理生理学教研室

出　　处：《中国病理生理杂志》1999年第3期197-199,共3页Chinese Journal of Pathophysiology

基　　金：国家八五攻关项目

摘　　要：目的与方法：应用分子杂交和放免法观察了缺氧下培养的猪肺动脉内皮细胞（ＰＡＥＣ）和冠状动脉内皮细胞（ＣＡＥＣ）的环氧合酶－１（ＣＯＸ－１），环氧合酶－２（ＣＯＸ－２）和血栓素Ａ２合酶（ＴＸＳ）基因表达及前列腺素生成的变化。结果：（１）常氧下培养２４ｈＰＡＥＣ的ＣＯＸ－２ｍＲＮＡ含量为ＣＡＥＣ的１５倍；ＰＡＥＣ的ＰＧＩ２生成量明显高于ＣＡＥＣ。（２）缺氧培养２４ｈＰＡＥＣ和ＣＡＥＣ中ＣＯＸ－１ｍＲＮＡ均增加，分别是其常氧对照组的２５倍和３倍，而ＣＯＸ－２ｍＲＮＡ含量仅在ＰＡＥＣ中明显增多，达其对照组的１６倍。（３）缺氧对ＣＡＥＣ的ＰＧＩ２生成量增加的幅度大于ＰＡＥＣ的增量。（４）ＰＡＥＣ和ＣＡＥＣ在常氧下和缺氧时ＴＸＳ基因表达ＴＸＡ２产量均无明显差异。AIM:The expression of cyclooxygenase-1 (COX-1), cyclooxygenase-2(COX-2) as well as thromboxane synthase (TXS) genes and the production of PGI 2 and TXA 2 in cultured pulmonary and coronary artery endothelial cells (PAEC and CAEC) were studied by methods of dot blot and IA.RESULTS:(1)After cultured in normoxia for 24h, COX-2 mRNA in PAEC was 1.5 times as much as those in CAEC( P <0.05); (2)After cultured in hypoxia for 24 h, COX-1 mRNA were 2.5 times in PAEC and 3 times in CAEC compared with normoxic control. However, COX-2 mRNA only increased in hypoxic PAEC, which was 1.6 times that of it's normoxic group; (3) Hypoxia increased the production of PGI 2 both in PAEC and CAEC, its increment in PAEC was higher than that in CAEC;(4) There was no difference in TXS gene expression and TXA 2 production of PAEC and CAEC either in normoxic or in hypoxic group.CONCLUSION:The difference in COX gene expression and PGI 2 production between PAEC and CAEC might be responsible for their different basal tone and their different responsiveness to hypoxia.

关 键 词：肺动脉 冠状动脉 内皮 环氧合酶 缺氧 TXA2 

分 类 号：R543.02[医药卫生—心血管疾病] R594.302[医药卫生—内科学]