含氮功能基高分子胶乳的研制及其在多种疾病快速诊断中的应用  

Preparation of Latices with Functional Group Containing N and Their Application in Rapid Diagnosis of Some Diseases

作　　者：吴道澄[1] 刘有初[1] 赵小宁[1] 田小东[2] 铙颐年 

机构地区：[1]第四军医大学化学教研室,西安710032 [2]广州军区军事医学研究所,广州510507

出　　处：《生物医学工程学杂志》1999年第1期1-4,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目;全军八五攻关课题

摘　　要：报告近年来我们研制的含有氮功能基（ＮＨ２、ＣＯＮ３ 等）的高分子胶乳载体的制备、性质及其在疾病快速诊断中的应用。即重氮化聚苯乙烯胶乳，三元共聚重氮化胶乳、叠氮胶乳及细胞标记用彩色高分子胶乳。用三元共聚重氮胶乳制备了登革病毒免疫快诊试剂，其检出敏感性达８２．３％ ，且５ ｍ ｉｎ 即出结果；检测登革热病人急性期血清标本４０ 例；同时与ＰＣＲ（聚合酶链反应）及病毒分离方法进行比较，结果表明三种方法的联合检出率为９２．５％ ，而胶乳凝集法的敏感性与ＰＣＲ 类似（７５％ ），高于病毒分离方法（５５％ ），但时间仅为ＰＣＲ 法的几十分之一。用叠氮胶乳检测类风湿因子、ＨＣＧ 抗体及登革病毒也取得了较好的检验效果。This article reports the preparation and diagnostic application of a variety of latices. These latices, including temary copolymer diazo latex, azidocarbonyl latex and cell labeling latex are stable, monodisperse, soap free, and covalently combined with protein in the absence of condensing agent. We have improved diazo polystyrene latex and made ternary copolymer diazo latex for early and rapid diagnosis of some diseases. Immunoassay reagent for the dectection of dengue fever has been prepared by our lab using ternary copolymeric diazo latex and azidocarbonyl latex. The experiment showed virus antigen could be detected in 5 mins by using diazo diagnostic agent. The sensitivity of detecting patient serum sample was 82.25%; no across reaction was observed in other inserted spread virus antigens and in serum of other feverish patient and normal control. 40 serum samples were dectected by latex agglutination assay using ternary copolymeric diazo latex immunoassay reagent. The results were compared with those of PCR and virus separation methods.The sensitivities of the three methods were 82.25%, 75% and 55% respectively; the combined sensitivity was 92.5%; higher sensitivities could be obtained by the former two methods within 1~13 days after the onset of disease.

关 键 词：氮功能基 高分子胶乳 快速诊断 登革热 

分 类 号：R512.804[医药卫生—内科学] R446[医药卫生—临床医学]