谷氨酰胺减轻烧伤大鼠心肌损害的研究  

Effects of glutamine on promoting protective protein synthesis and lessening myocardial injury of burned rats

作　　者：吕尚军[1] 吴炜[1] 张勇[1] 孙勇[1] 王林[1] 王臻[1] 彭曦[1] 

机构地区：[1]第三军医大学西南医院解放军烧伤研究所创伤烧伤与复合伤研究国家重点实验室,重庆400038

出　　处：《肠外与肠内营养》2010年第4期236-238,242,共4页Parenteral & Enteral Nutrition

基　　金：国家重点基础发展计划项目资助(2005CB522601);国家自然科学基金(30670773)

摘　　要：目的:观察给予谷氨酰胺(Gln)以减轻严重烧伤大鼠心肌损害的作用,并探讨其机制。方法:将72只大鼠随机分为正常对照组、烧伤对照组和Gln组。采用30%体表面积(TBSA)Ⅲ度烧伤大鼠模型,Gln组给予Gln 1.0 g/(kg.d),烧伤对照组给予等量的酪氨酸,确保两组等氮和等热量。烧伤后12、24、48和72 h观察大鼠心肌组织形态学变化,同时检测心肌酶谱(CK、LDH、AST),心肌组织热休克蛋白70(HSP 70)和金属硫蛋白(MT)含量的变化。结果:Gln组大鼠心肌组织病理损伤程度较烧伤对照组明显减轻,血清CK、LDH和AST检测值也明显低于烧伤对照组(P<0.01),心肌组织HSP 70和MT含量高于烧伤对照组(P<0.01～0.05)。结论:大鼠烧伤后给予Gln,能促进心肌组织保护蛋白HSP70和MT的合成,减轻心肌损害程度。Objective ： To observe the effects of glutamine on promoting protective protein synthesis and lessening myocardial injury of burned rats, and explore its mechanism. Methods ： 72 rats were randomly divided into control group （ C, n = 8 ）, burned group （ B, n = 32 ）, glutamine group （Gln, n = 32）. The rats in GLN and B group were supplemented 1.0 g/（kg · d）glutamine or tyrosine respectively, after inflicted with 30% TBSA full-thickness burn on the back. The myocardium histopathological change, creatine kinase （ CK）, lactate dehydrogenase （ LDH ）, aspartate aminotransferase （ AST）, tissue heat shock protein 70（ HSP 70）and metallothionein（MT） were measured at 12,24,48,72 post burn hour（PBH）. Results： The content of CK, LDH, AST in Gin group were obviously lower than those in B group （P 〈 0.01 ）. Administration of Gin could significantly promote MT and HSP 70 protein synthesis and obviously lessen myocardial tissue pathological lesion when compared with B group（ P 〈 0. 01 -0.05 ）. Conclusion ： Gin can promote HSP 70 and MT synthesis and minimize myocardial damage after burn injury.

关 键 词：谷氨酰胺 热休克蛋白 金属硫蛋白 心肌 烧伤 

分 类 号：R644[医药卫生—外科学]